Cloned (Comment) | Organism |
---|---|
gene NSun2, recombinant expression of wild-type and mutant enzymes | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an insertion mutation in Drosophila NSun2 (NSun2DELTA21.5), which is viable and fertile and loses RNA methylation at known tRNA substrates. RNAi-mediated knockdown of NSun2 in follicle cells. Functional Gypsy retroviral particles can be formed in NSun2 mutants. Specific eccDNAs accumulate in RCMT mutants. RCMT mutants display reduced tRNA stability and tRNA abundance | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine34 in tRNA precursor | Drosophila melanogaster | - |
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9W4M9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovarian follicle | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine34 in tRNA precursor | - |
Drosophila melanogaster | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytosine-5 tRNA methyltransferase | - |
Drosophila melanogaster |
NSUN2 | - |
Drosophila melanogaster |
RCMT | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation in (cytosine-5) RNA methyltransferase NSun2, which targets mostly tRNAs, impacts the expression of mobile element-derived sequences and affects DNA repeat integrity in Drosophila melanogaster. Reduced tRNA stability in the RCMT mutant indicates that tRNA-dependent processes affect mobile element expression and DNA repeat stability. NSun2 function affects mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in both RCMT mutants indicates that tRNA-dependent processes affect mobile element expression and DNA repeat stability. NSun2 mutants show heat-shock-independent transposable element (TE) expression changes | Drosophila melanogaster |
physiological function | mutations in two (cytosine-5) RNA methyltransferase NSun2 impact the accumulation of mobile element-derived sequences and DNA repeat integrity in Drosophila melanogaster. NSun2 function affects mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in mutants indicates that tRNA-dependent processes affected mobile element expression and DNA repeat stability | Drosophila melanogaster |
physiological function | NSun2 protein is a highly conserved (cytosine-5) methyltransferases that methylate specific tRNAs instead of genomic DNA. NSun2 function affects repeat elements in a heat shock-independent fashion. NSun2, is an RCMT targeting the majority of tRNAs | Drosophila melanogaster |