BRENDA - Enzyme Database show
show all sequences of 2.1.1.202

TRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae

Preston, M.; DSilva, S.; Kon, Y.; Phizicky, E.; RNA 19, 243-256 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae BY4741
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine34 in tRNA
Saccharomyces cerevisiae BY4741
cf. EC 2.1.1.203
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
Saccharomyces cerevisiae BY4741
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine34 in tRNA
cf. EC 2.1.1.203
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAHis
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
-
737227
Saccharomyces cerevisiae BY4741
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
-
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAHis
purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview
737227
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis
-
-
-
?
Expression
Organism
Commentary
Expression
Saccharomyces cerevisiae
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
additional information
General Information
General Information
Commentary
Organism
malfunction
yeast strains depleted of tRNAHis guanylyltransferase accumulate uncharged tRNAHis lacking the G-1 residue and subsequently accumulate additional 5-methylcytidine (m5C) at residues C48 and C50 of tRNAHis, due to the activity of the m5Cmethyltransferase Trm4. The increase in tRNAHis m5C levels does not require loss of Thg1, loss of G-1 of tRNAHis, or cell death but is associated with growth arrest following different stress conditions. Substantially increased tRNAHis m5C levels occur after temperature-sensitive strains are grown at nonpermissive temperature, and after wild-type strains are grown to stationary phase, starved for required amino acids, or treated with rapamycin. More modest accumulations of m5C in tRNAHis occur after starvation for glucose and after starvation for uracil. In virtually all cases examined, the additional m5C on tRNAHis occurs while cells are fully viable, and the increase is neither due to the GCN4 pathway, nor to increased Trm4 levels, phenotypes, overview. The increased amount of m5C is specific to tRNAHis. tRNAVal(AAC), which also normally has unmodified C48 and C50 residues adjacent to m5C49, has only marginally increased levels of m5C 7 h after temperature shift in the fcp1-1ts mutant
Saccharomyces cerevisiae
metabolism
the GCN4 pathway is not responsible for additional m5C levels on tRNAHis
Saccharomyces cerevisiae
additional information
tRNAHis m5C levels are unusually responsive to yeast growth conditions
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
yeast strains depleted of tRNAHis guanylyltransferase accumulate uncharged tRNAHis lacking the G-1 residue and subsequently accumulate additional 5-methylcytidine (m5C) at residues C48 and C50 of tRNAHis, due to the activity of the m5Cmethyltransferase Trm4. The increase in tRNAHis m5C levels does not require loss of Thg1, loss of G-1 of tRNAHis, or cell death but is associated with growth arrest following different stress conditions. Substantially increased tRNAHis m5C levels occur after temperature-sensitive strains are grown at nonpermissive temperature, and after wild-type strains are grown to stationary phase, starved for required amino acids, or treated with rapamycin. More modest accumulations of m5C in tRNAHis occur after starvation for glucose and after starvation for uracil. In virtually all cases examined, the additional m5C on tRNAHis occurs while cells are fully viable, and the increase is neither due to the GCN4 pathway, nor to increased Trm4 levels, phenotypes, overview. The increased amount of m5C is specific to tRNAHis. tRNAVal(AAC), which also normally has unmodified C48 and C50 residues adjacent to m5C49, has only marginally increased levels of m5C 7 h after temperature shift in the fcp1-1ts mutant
Saccharomyces cerevisiae
metabolism
the GCN4 pathway is not responsible for additional m5C levels on tRNAHis
Saccharomyces cerevisiae
additional information
tRNAHis m5C levels are unusually responsive to yeast growth conditions
Saccharomyces cerevisiae
Expression (protein specific)
Organism
Commentary
Expression
Saccharomyces cerevisiae
levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions
additional information
Other publictions for EC 2.1.1.202
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana, Brassica rapa, Caulerpa taxifolia, Ginkgo biloba, Nannochloropsis oculata, Triticum turgidum subsp. durum
BMC Plant Biol.
15
199
2015
-
-
1
-
-
-
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1
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6
-
19
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1
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7
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6
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1
6
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1
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6
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1
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7
-
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-
-
-
-
-
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-
13
13
-
-
-
735680
Moon
Trm4 and Nsun2 RNA:m5C methylt ...
Saccharomyces cerevisiae
Biochemistry
53
7132-7144
2014
-
-
-
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-
-
-
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1
-
2
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2
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1
-
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1
-
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1
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1
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-
-
2
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1
-
-
-
-
2
2
-
-
-
735907
Hussain
NSun2-mediated cytosine-5 meth ...
Homo sapiens
Cell Rep.
4
255-261
2013
-
-
-
-
2
-
-
-
-
-
-
10
-
1
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1
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11
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1
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1
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2
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10
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1
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-
11
-
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-
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1
2
2
1
-
-
736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
1
-
2
-
1
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8
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1
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4
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1
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9
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1
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1
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1
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2
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1
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8
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1
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9
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2
2
-
-
-
737227
Preston
TRNAHis 5-methylcytidine level ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
RNA
19
243-256
2013
1
-
-
-
-
-
-
-
-
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11
-
58
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13
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1
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1
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1
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11
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-
13
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
719968
Awai
Substrate tRNA recognition mec ...
Methanocaldococcus jannaschii
J. Biol. Chem.
286
35236-35246
2011
-
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1
1
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2
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1
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1
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3
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1
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1
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2
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1
2
1
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2
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1
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3
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1
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-
1
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-
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-
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-
710546
Walbott
Cysteine of sequence motif VI ...
Saccharomyces cerevisiae
RNA
13
967-973
2007
-
-
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1
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3
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1
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1
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1
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672512
Redman
Assembly of protein-RNA comple ...
Saccharomyces cerevisiae
Biomacromolecules
7
3321-3326
2006
-
-
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2
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2
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2
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710135
Brzezicha
Identification of human tRNA:m ...
Saccharomyces cerevisiae
Nucleic Acids Res.
34
6034-6043
2006
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1
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3
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3
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1
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1
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1
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-
-
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3
-
1
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-
1
-
-
-
-
-
-
-
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-
639472
King
RNA methyltransferases utilize ...
Saccharomyces cerevisiae
Biochemistry
41
11218-11225
2002
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2
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3
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1
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2
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1
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639471
Motorin
Multisite-specific tRNA:m5C-me ...
Saccharomyces cerevisiae
RNA
5
1105-1118
1999
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1
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3
4
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4
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1
4
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8
1
1
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1
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1
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3
4
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1
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1
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1997
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4
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1
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1
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4
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1
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1
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Strobel
Effect of intron mutations on ...
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1986
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