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Literature summary for 2.1.1.202 extracted from
- TRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae (2013), RNA, 19, 243-256.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine34 in tRNA | Saccharomyces cerevisiae | cf. EC 2.1.1.203 | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA | - |
? |  |
| S-adenosyl-L-methionine + cytosine34 in tRNA | Saccharomyces cerevisiae BY4741 | cf. EC 2.1.1.203 | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine40 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine40 in tRNA | Saccharomyces cerevisiae BY4741 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNA | Saccharomyces cerevisiae BY4741 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNAHis | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNAHis | Saccharomyces cerevisiae BY4741 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine49 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine49 in tRNA | Saccharomyces cerevisiae BY4741 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine50 in tRNAHis | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae BY4741 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine34 in tRNA | cf. EC 2.1.1.203 | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine34 in tRNA | cf. EC 2.1.1.203 | Saccharomyces cerevisiae BY4741 | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine40 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine40 in tRNA | - |
Saccharomyces cerevisiae BY4741 | S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNA | - |
Saccharomyces cerevisiae BY4741 | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNAHis | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNAHis | purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine48 in tRNAHis | - |
Saccharomyces cerevisiae BY4741 | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine49 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine49 in tRNA | - |
Saccharomyces cerevisiae BY4741 | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine50 in tRNAHis | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis | - |
? | |
| S-adenosyl-L-methionine + cytosine50 in tRNAHis | purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TRM4 | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | levels of tRNAHis m5C increase following several nutrient starvation treatments of the BY4741 strain, Trm4 protein levels do not increase during starvation conditions | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | yeast strains depleted of tRNAHis guanylyltransferase accumulate uncharged tRNAHis lacking the G-1 residue and subsequently accumulate additional 5-methylcytidine (m5C) at residues C48 and C50 of tRNAHis, due to the activity of the m5Cmethyltransferase Trm4. The increase in tRNAHis m5C levels does not require loss of Thg1, loss of G-1 of tRNAHis, or cell death but is associated with growth arrest following different stress conditions. Substantially increased tRNAHis m5C levels occur after temperature-sensitive strains are grown at nonpermissive temperature, and after wild-type strains are grown to stationary phase, starved for required amino acids, or treated with rapamycin. More modest accumulations of m5C in tRNAHis occur after starvation for glucose and after starvation for uracil. In virtually all cases examined, the additional m5C on tRNAHis occurs while cells are fully viable, and the increase is neither due to the GCN4 pathway, nor to increased Trm4 levels, phenotypes, overview. The increased amount of m5C is specific to tRNAHis. tRNAVal(AAC), which also normally has unmodified C48 and C50 residues adjacent to m5C49, has only marginally increased levels of m5C 7 h after temperature shift in the fcp1-1ts mutant | Saccharomyces cerevisiae |
| metabolism | the GCN4 pathway is not responsible for additional m5C levels on tRNAHis | Saccharomyces cerevisiae |
| additional information | tRNAHis m5C levels are unusually responsive to yeast growth conditions | Saccharomyces cerevisiae |
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