Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA-mediated enzyme knockout in HeLa cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aza-2'-deoxycytidine | the catalytic mechanism is disrupted by the suicide inhibitor | Homo sapiens | |
5-azacytidine | the catalytic mechanism is disrupted by the suicide inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleolus | - |
Homo sapiens | 5730 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides | ? | - |
? | |
S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNALeu precursor | - |
? | |
S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp precursor | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly precursor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor | the enzyme forms a covalent enzyme-substrate intermediate with its target | Homo sapiens | |
S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor | the enzyme forms a covalent enzyme-substrate intermediate with its target | Homo sapiens | |
S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA | the enzyme forms a covalent enzyme-substrate intermediate with its target | Homo sapiens | |
S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA | the enzyme forms a covalent enzyme-substrate intermediate with its target | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides | Homo sapiens | ? | - |
? | |
additional information | development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. The method enables over 200fold enrichment of tRNAs that are known targets of the enzyme revealing many tRNA and non-coding RNA targets not previously associated with NSUN2. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC), bears four NSUN2 target sites (C40, 48, 49 and 50) | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNALeu precursor | - |
? | |
S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp precursor | - |
? | |
S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp precursor | - |
? | |
S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly precursor | - |
? | |
S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly precursor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NSUN2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the RsmF/YebU/NSUN2 family of cytosine 5-methylation-RNA methyltransferases utilizing two cysteines in their catalytic pocket | Homo sapiens |
malfunction | NSUN2 is associated with Myc-induced proliferation of cancer cells, mitotic spindle stability, infertility in male mice, and the balance of selfrenewal and differentiation in skin stem cells. In humans NSUN2 mutations cause an autosomal recessive syndrome characterized by intellectual disability and mental retardation | Homo sapiens |