BRENDA - Enzyme Database show
show all sequences of 2.1.1.202

NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs

Hussain, S.; Sajini, A.A.; Blanco, S.; Dietmann, S.; Lombard, P.; Sugimoto, Y.; Paramor, M.; Gleeson, J.G.; Odom, D.T.; Ule, J.; Frye, M.; Cell Rep. 4, 255-261 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C271A
site-directed mutagenesis, the mutant enzyme forms an irreversible covalent crosslink between itself and the methylated cytosine in its target RNA
Homo sapiens
additional information
the only mRNA identified by miCLIP that is differentially expressed when NSun2 is inhibited by RNAi in HEK293 cells is NSun2 itself
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex
?
-
-
-
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
skin fibroblast
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex
735907
Homo sapiens
?
-
-
-
-
additional information
development and evaluation of a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method for detection of cytosine methylation in RNA species, site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs), overview. Identified NSun2 targets are tRNAs, mRNAs, and ncRNAs
735907
Homo sapiens
?
-
-
-
-
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C271A
site-directed mutagenesis, the mutant enzyme forms an irreversible covalent crosslink between itself and the methylated cytosine in its target RNA
Homo sapiens
additional information
the only mRNA identified by miCLIP that is differentially expressed when NSun2 is inhibited by RNAi in HEK293 cells is NSun2 itself
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex
?
-
-
-
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
skin fibroblast
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex
735907
Homo sapiens
?
-
-
-
-
additional information
development and evaluation of a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method for detection of cytosine methylation in RNA species, site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs), overview. Identified NSun2 targets are tRNAs, mRNAs, and ncRNAs
735907
Homo sapiens
?
-
-
-
-
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA
-
735907
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
-
-
?
Expression
Organism
Commentary
Expression
Homo sapiens
sequenced cDNA libraries prepared from small RNA isolated from NSUN2+/- and NSUN2-/- human fibroblasts reveal that eight of the 43 ncRNAs are differentially regulated
additional information
General Information
General Information
Commentary
Organism
malfunction
autosomal-recessive loss of the NSUN2 gene is a causative link to intellectual disability disorders in humans. Loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders
Homo sapiens
physiological function
no evidence for a major role of NSun2 or NSun2-mediated cytosine-5 methylation in mRNA stability
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
autosomal-recessive loss of the NSUN2 gene is a causative link to intellectual disability disorders in humans. Loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders
Homo sapiens
physiological function
no evidence for a major role of NSun2 or NSun2-mediated cytosine-5 methylation in mRNA stability
Homo sapiens
Expression (protein specific)
Organism
Commentary
Expression
Homo sapiens
sequenced cDNA libraries prepared from small RNA isolated from NSUN2+/- and NSUN2-/- human fibroblasts reveal that eight of the 43 ncRNAs are differentially regulated
additional information
Other publictions for EC 2.1.1.202
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana, Brassica rapa, Caulerpa taxifolia, Ginkgo biloba, Nannochloropsis oculata, Triticum turgidum subsp. durum
BMC Plant Biol.
15
199
2015
-
-
1
-
-
-
-
-
1
-
-
6
-
19
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
6
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
13
13
-
-
-
735680
Moon
Trm4 and Nsun2 RNA:m5C methylt ...
Saccharomyces cerevisiae
Biochemistry
53
7132-7144
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
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-
-
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-
1
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1
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-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
735907
Hussain
NSun2-mediated cytosine-5 meth ...
Homo sapiens
Cell Rep.
4
255-261
2013
-
-
-
-
2
-
-
-
-
-
-
10
-
1
-
-
-
-
-
1
-
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
-
-
11
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
1
-
2
-
1
-
-
8
-
1
-
-
-
4
-
1
-
-
9
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
1
-
-
8
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
737227
Preston
TRNAHis 5-methylcytidine level ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
RNA
19
243-256
2013
1
-
-
-
-
-
-
-
-
-
-
11
-
58
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
719968
Awai
Substrate tRNA recognition mec ...
Methanocaldococcus jannaschii
J. Biol. Chem.
286
35236-35246
2011
-
-
1
1
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
2
-
-
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1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
710546
Walbott
Cysteine of sequence motif VI ...
Saccharomyces cerevisiae
RNA
13
967-973
2007
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
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-
-
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
672512
Redman
Assembly of protein-RNA comple ...
Saccharomyces cerevisiae
Biomacromolecules
7
3321-3326
2006
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
-
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2
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-
-
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-
-
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-
710135
Brzezicha
Identification of human tRNA:m ...
Saccharomyces cerevisiae
Nucleic Acids Res.
34
6034-6043
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
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1
-
-
-
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1
-
-
-
-
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-
-
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-
-
-
-
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-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639472
King
RNA methyltransferases utilize ...
Saccharomyces cerevisiae
Biochemistry
41
11218-11225
2002
-
-
-
-
2
-
-
-
-
-
-
-
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3
-
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1
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2
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1
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-
-
-
-
-
-
-
-
-
-
-
639471
Motorin
Multisite-specific tRNA:m5C-me ...
Saccharomyces cerevisiae
RNA
5
1105-1118
1999
-
-
1
-
-
-
-
-
-
-
3
4
-
4
-
-
1
4
-
-
-
-
8
1
1
-
-
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
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-
3
4
-
-
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1
-
-
-
-
8
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
710134
Jiang
Pleiotropic effects of intron ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae Pp1001
Nucleic Acids Res.
25
2694-2701
1997
-
-
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-
-
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-
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2
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4
-
1
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1
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-
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-
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-
-
-
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-
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4
-
1
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1
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-
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-
-
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-
709946
Strobel
Effect of intron mutations on ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
6
2663-2673
1986
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