BRENDA - Enzyme Database show
show all sequences of 2.1.1.202

Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study

Jiang, H.Q.; Motorin, Y.; Jin, Y.X.; Grosjean, H.; Nucleic Acids Res. 25, 2694-2701 (1997)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae Pp1001
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine40 in tRNA precursor
the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron
710134
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA precursor
the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron
710134
Saccharomyces cerevisiae Pp1001
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNA(GAA)
710134
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNA(GAA)
710134
Saccharomyces cerevisiae Pp1001
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine40 in tRNA precursor
the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron
710134
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine40 in tRNA precursor
the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron
710134
Saccharomyces cerevisiae Pp1001
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNA(GAA)
710134
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine49 in tRNA
yeast tRNA(GAA)
710134
Saccharomyces cerevisiae Pp1001
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 2.1.1.202
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana, Brassica rapa, Caulerpa taxifolia, Ginkgo biloba, Nannochloropsis oculata, Triticum turgidum subsp. durum
BMC Plant Biol.
15
199
2015
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1
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1
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6
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19
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1
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7
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6
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1
6
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1
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6
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1
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7
-
-
-
-
-
-
-
-
-
-
13
13
-
-
-
735680
Moon
Trm4 and Nsun2 RNA:m5C methylt ...
Saccharomyces cerevisiae
Biochemistry
53
7132-7144
2014
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-
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1
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2
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2
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1
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1
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1
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1
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-
2
-
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1
-
-
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-
2
2
-
-
-
735907
Hussain
NSun2-mediated cytosine-5 meth ...
Homo sapiens
Cell Rep.
4
255-261
2013
-
-
-
-
2
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-
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-
-
10
-
1
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1
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11
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1
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1
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2
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10
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1
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-
11
-
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-
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-
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1
2
2
1
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-
736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
1
-
2
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1
-
-
8
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1
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-
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4
-
1
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9
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1
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1
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1
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2
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1
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8
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1
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9
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2
2
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737227
Preston
TRNAHis 5-methylcytidine level ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
RNA
19
243-256
2013
1
-
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-
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-
11
-
58
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13
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1
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1
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1
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11
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-
13
-
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-
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-
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1
3
3
1
-
-
719968
Awai
Substrate tRNA recognition mec ...
Methanocaldococcus jannaschii
J. Biol. Chem.
286
35236-35246
2011
-
-
1
1
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2
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1
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1
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3
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1
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1
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2
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1
2
1
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2
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1
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3
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1
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1
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710546
Walbott
Cysteine of sequence motif VI ...
Saccharomyces cerevisiae
RNA
13
967-973
2007
-
-
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1
-
-
-
-
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-
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3
-
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1
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1
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1
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672512
Redman
Assembly of protein-RNA comple ...
Saccharomyces cerevisiae
Biomacromolecules
7
3321-3326
2006
-
-
-
-
2
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-
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2
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2
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710135
Brzezicha
Identification of human tRNA:m ...
Saccharomyces cerevisiae
Nucleic Acids Res.
34
6034-6043
2006
-
-
1
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3
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3
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1
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1
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1
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3
-
1
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1
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-
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-
-
639472
King
RNA methyltransferases utilize ...
Saccharomyces cerevisiae
Biochemistry
41
11218-11225
2002
-
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2
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-
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3
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1
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2
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1
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639471
Motorin
Multisite-specific tRNA:m5C-me ...
Saccharomyces cerevisiae
RNA
5
1105-1118
1999
-
-
1
-
-
-
-
-
-
-
3
4
-
4
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1
4
-
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8
1
1
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1
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1
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3
4
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1
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8
1
1
-
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-
1
-
-
-
-
2
2
-
-
-
710134
Jiang
Pleiotropic effects of intron ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae Pp1001
Nucleic Acids Res.
25
2694-2701
1997
-
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2
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4
-
1
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1
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4
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1
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1
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-
709946
Strobel
Effect of intron mutations on ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
6
2663-2673
1986
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1
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1
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