Literature summary for 2.1.1.201 extracted from

Barkovich, R.J.; Shtanko, A.; Shepherd, J.A.; Lee, P.T.; Myles, D.C.; Tzagoloff, A.; Clarke, C.F.
Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis (1997), J. Biol. Chem., 272, 9182-918.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34685	-	x * 34685, calculated from sequence	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 2-methoxy-6-(all-trans-hexaprenyl)-1,4-benzoquinol	Saccharomyces cerevisiae	-	S-adenosyl-L-homocysteine + 6-methoxy-5-methyl-2-(all-trans)-hexaprenyl-1,4-benzoquinol	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P49017	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 2-methoxy-6-(all-trans-hexaprenyl)-1,4-benzoquinol	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 6-methoxy-5-methyl-2-(all-trans)-hexaprenyl-1,4-benzoquinol	-	?
S-adenosyl-L-methionine + 2-methoxy-6-polyprenyl-1,4-benzoquinol	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 6-methoxy-5-methyl-2-polyprenyl-1,4-benzoquinol	-	?
S-adenosyl-L-methionine + 6-((2-trans,6-trans)-farnesyl)-2-methoxy-1,4-benzoquinone	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 6-((2-trans,6-trans)-farnesyl)-5-methyl-2-methoxy-1,4-benzoquinone	product determined by reverse-phase HPLC	?

Subunits

Subunits	Comment	Organism
?	x * 34685, calculated from sequence	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
COQ5	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
NADH	NAFH is required form optimal activity in in vitro assay with mitochondrial lysate. It is likely that the function of NADH is the reduction of the oxidized 2-methoxy-6-polyprenyl-1,4-benzoquinone substrate to the hydroquinone form (2-methoxy-6-polyprenyl-1,4-benzoquinol), which presumably acts as the methyl acceptor	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)