Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y21A | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
Y21F | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
Y21G | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
Y21S | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
Y21T | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
Y21V | mutant yields the same magnitude of binary isotope effect as wild-type | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
S-adenosyl-L-methionine | wild-type, pH 7.4, 37°C | Rattus norvegicus | |
0.174 | - |
S-adenosyl-L-methionine | mutant Y21F, pH 7.4, 37°C | Rattus norvegicus | |
0.539 | - |
S-adenosyl-L-methionine | mutant Y21A, pH 7.4, 37°C | Rattus norvegicus | |
0.582 | - |
S-adenosyl-L-methionine | mutant Y21V, pH 7.4, 37°C | Rattus norvegicus | |
0.696 | - |
S-adenosyl-L-methionine | mutant Y21G, pH 7.4, 37°C | Rattus norvegicus | |
0.803 | - |
S-adenosyl-L-methionine | mutant Y21S, pH 7.4, 37°C | Rattus norvegicus | |
1.1 | - |
glycine | wild-type, pH 7.4, 37°C | Rattus norvegicus | |
1.136 | - |
S-adenosyl-L-methionine | mutant Y21T, pH 7.4, 37°C | Rattus norvegicus | |
1.7 | - |
glycine | mutant Y21F, pH 7.4, 37°C | Rattus norvegicus | |
2 | 3.4 | glycine | mutant Y21S, pH 7.4, 37°C | Rattus norvegicus | |
22.9 | - |
glycine | mutant Y21T, pH 7.4, 37°C | Rattus norvegicus | |
26.6 | - |
glycine | mutant Y21A, pH 7.4, 37°C | Rattus norvegicus | |
33.6 | - |
glycine | mutant Y21G, pH 7.4, 37°C | Rattus norvegicus | |
38.8 | - |
glycine | mutant Y21V, pH 7.4, 37°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P13255 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | kinetic isotope effects at the transferred methyl group implicate a compaction effect that is conferred by the protein structure | Rattus norvegicus | ? | - |
- |
|
S-adenosyl-L-methionine + glycine | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + sarcosine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0097 | - |
S-adenosyl-L-methionine | mutant Y21V, pH 7.4, 37°C | Rattus norvegicus | |
0.0098 | - |
S-adenosyl-L-methionine | mutant Y21A, pH 7.4, 37°C | Rattus norvegicus | |
0.0108 | - |
S-adenosyl-L-methionine | mutant Y21S, pH 7.4, 37°C | Rattus norvegicus | |
0.0135 | - |
S-adenosyl-L-methionine | mutant Y21T, pH 7.4, 37°C | Rattus norvegicus | |
0.0155 | - |
S-adenosyl-L-methionine | mutant Y21G, pH 7.4, 37°C | Rattus norvegicus | |
0.42 | - |
S-adenosyl-L-methionine | mutant Y21F, pH 7.4, 37°C | Rattus norvegicus | |
2.908 | - |
S-adenosyl-L-methionine | wild-type, pH 7.4, 37°C | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
glycine | mutant Y21V, pH 7.4, 37°C | Rattus norvegicus | |
0.0004 | - |
glycine | mutant Y21A, pH 7.4, 37°C | Rattus norvegicus | |
0.0005 | - |
glycine | mutant Y21G, pH 7.4, 37°C | Rattus norvegicus | |
0.0005 | - |
glycine | mutant Y21S, pH 7.4, 37°C | Rattus norvegicus | |
0.0006 | - |
glycine | mutant Y21T, pH 7.4, 37°C | Rattus norvegicus | |
0.012 | - |
S-adenosyl-L-methionine | mutant Y21T, pH 7.4, 37°C | Rattus norvegicus | |
0.014 | - |
S-adenosyl-L-methionine | mutant Y21S, pH 7.4, 37°C | Rattus norvegicus | |
0.017 | - |
S-adenosyl-L-methionine | mutant Y21V, pH 7.4, 37°C | Rattus norvegicus | |
0.018 | - |
S-adenosyl-L-methionine | mutant Y21A, pH 7.4, 37°C | Rattus norvegicus | |
0.022 | - |
S-adenosyl-L-methionine | mutant Y21G, pH 7.4, 37°C | Rattus norvegicus | |
0.248 | - |
glycine | mutant Y21F, pH 7.4, 37°C | Rattus norvegicus | |
2.409 | - |
S-adenosyl-L-methionine | mutant Y21F, pH 7.4, 37°C | Rattus norvegicus | |
2.616 | - |
glycine | wild-type, pH 7.4, 37°C | Rattus norvegicus | |
26.935 | - |
S-adenosyl-L-methionine | wild-type, pH 7.4, 37°C | Rattus norvegicus |