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Literature summary for 2.1.1.20 extracted from

  • Zhang, J.; Klinman, J.P.
    Convergent mechanistic features between the structurally diverse N- and O-methyltransferases glycine N-methyltransferase and catechol O-methyltransferase (2016), J. Am. Chem. Soc., 138, 9158-9165 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
Y21A mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus
Y21F mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus
Y21G mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus
Y21S mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus
Y21T mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus
Y21V mutant yields the same magnitude of binary isotope effect as wild-type Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.108
-
S-adenosyl-L-methionine wild-type, pH 7.4, 37°C Rattus norvegicus
0.174
-
S-adenosyl-L-methionine mutant Y21F, pH 7.4, 37°C Rattus norvegicus
0.539
-
S-adenosyl-L-methionine mutant Y21A, pH 7.4, 37°C Rattus norvegicus
0.582
-
S-adenosyl-L-methionine mutant Y21V, pH 7.4, 37°C Rattus norvegicus
0.696
-
S-adenosyl-L-methionine mutant Y21G, pH 7.4, 37°C Rattus norvegicus
0.803
-
S-adenosyl-L-methionine mutant Y21S, pH 7.4, 37°C Rattus norvegicus
1.1
-
glycine wild-type, pH 7.4, 37°C Rattus norvegicus
1.136
-
S-adenosyl-L-methionine mutant Y21T, pH 7.4, 37°C Rattus norvegicus
1.7
-
glycine mutant Y21F, pH 7.4, 37°C Rattus norvegicus
2 3.4 glycine mutant Y21S, pH 7.4, 37°C Rattus norvegicus
22.9
-
glycine mutant Y21T, pH 7.4, 37°C Rattus norvegicus
26.6
-
glycine mutant Y21A, pH 7.4, 37°C Rattus norvegicus
33.6
-
glycine mutant Y21G, pH 7.4, 37°C Rattus norvegicus
38.8
-
glycine mutant Y21V, pH 7.4, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P13255
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information kinetic isotope effects at the transferred methyl group implicate a compaction effect that is conferred by the protein structure Rattus norvegicus ?
-
-
S-adenosyl-L-methionine + glycine
-
Rattus norvegicus S-adenosyl-L-homocysteine + sarcosine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0097
-
S-adenosyl-L-methionine mutant Y21V, pH 7.4, 37°C Rattus norvegicus
0.0098
-
S-adenosyl-L-methionine mutant Y21A, pH 7.4, 37°C Rattus norvegicus
0.0108
-
S-adenosyl-L-methionine mutant Y21S, pH 7.4, 37°C Rattus norvegicus
0.0135
-
S-adenosyl-L-methionine mutant Y21T, pH 7.4, 37°C Rattus norvegicus
0.0155
-
S-adenosyl-L-methionine mutant Y21G, pH 7.4, 37°C Rattus norvegicus
0.42
-
S-adenosyl-L-methionine mutant Y21F, pH 7.4, 37°C Rattus norvegicus
2.908
-
S-adenosyl-L-methionine wild-type, pH 7.4, 37°C Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0003
-
glycine mutant Y21V, pH 7.4, 37°C Rattus norvegicus
0.0004
-
glycine mutant Y21A, pH 7.4, 37°C Rattus norvegicus
0.0005
-
glycine mutant Y21G, pH 7.4, 37°C Rattus norvegicus
0.0005
-
glycine mutant Y21S, pH 7.4, 37°C Rattus norvegicus
0.0006
-
glycine mutant Y21T, pH 7.4, 37°C Rattus norvegicus
0.012
-
S-adenosyl-L-methionine mutant Y21T, pH 7.4, 37°C Rattus norvegicus
0.014
-
S-adenosyl-L-methionine mutant Y21S, pH 7.4, 37°C Rattus norvegicus
0.017
-
S-adenosyl-L-methionine mutant Y21V, pH 7.4, 37°C Rattus norvegicus
0.018
-
S-adenosyl-L-methionine mutant Y21A, pH 7.4, 37°C Rattus norvegicus
0.022
-
S-adenosyl-L-methionine mutant Y21G, pH 7.4, 37°C Rattus norvegicus
0.248
-
glycine mutant Y21F, pH 7.4, 37°C Rattus norvegicus
2.409
-
S-adenosyl-L-methionine mutant Y21F, pH 7.4, 37°C Rattus norvegicus
2.616
-
glycine wild-type, pH 7.4, 37°C Rattus norvegicus
26.935
-
S-adenosyl-L-methionine wild-type, pH 7.4, 37°C Rattus norvegicus