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Literature summary for 2.1.1.20 extracted from

  • Luka, Z.; Wagner, C.
    Effect of naturally occurring mutations in human glycine N-methyltransferase on activity and conformation (2003), Biochem. Biophys. Res. Commun., 312, 1067-1072.
    View publication on PubMed
Search for more literature for 2.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes H176N, L49P and N140S, expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
H176N mutant enzyme possesses 75% activity of the wild-type enzyme Homo sapiens
L49P mutant enzyme possesses 10% activity of the wild-type enzyme Homo sapiens
N140S mutant enzyme possesses less than 5% activity of the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0122
-
 Gly pH 7.5, 25°C, wild-type enzyme Homo sapiens
0.02
-
 Gly pH 7.5, 25°C, mutant enzyme H176N Homo sapiens
0.0721
-
 Gly pH 7.5, 25°C, mutant enzyme L49P Homo sapiens
0.12
-
 S-adenosyl-L-methionine pH 7.5, 25°C, mutant enzyme L49P Homo sapiens
0.242
-
 S-adenosyl-L-methionine pH 7.5, 25°C, mutant enzyme H176N Homo sapiens
0.281
-
 S-adenosyl-L-methionine pH 7.5, 25°C, wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + glycine
-
 Homo sapiens S-adenosyl-L-homocysteine + sarcosine
-
 ?

Synonyms

Synonyms Comment Organism
GNMT
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 S-adenosyl-L-methionine pH 7.5, 25°C, mutant enzyme L49P Homo sapiens
0.35
-
 Gly pH 7.5, 25°C, mutant enzyme L49P Homo sapiens
1.19
-
 S-adenosyl-L-methionine pH 7.5, 25°C, mutant enzyme H176N Homo sapiens
1.19
-
 Gly pH 7.5, 25°C, mutant enzyme H176N Homo sapiens
1.6
-
 S-adenosyl-L-methionine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.6
-
 Gly pH 7.5, 25°C, wild-type enzyme Homo sapiens