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Literature summary for 2.1.1.198 extracted from
- Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA (2010), Nucleic Acids Res., 38, 1341-1352.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytidine1402 in 16S rRNA | Escherichia coli | the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome | S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P67087 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein with N-terminal 6His-tags | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytidine1402 in 16S rRNA | the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RsmI | - |
Escherichia coli |
| YraL | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme | Escherichia coli |
| physiological function | both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons | Escherichia coli |
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Release 2023.1 (January 2023)
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