BRENDA - Enzyme Database
show all sequences of 2.1.1.198

Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA

Kimura, S.; Suzuki, T.; Nucleic Acids Res. 38, 1341-1352 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
Escherichia coli
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P67087
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein with N-terminal 6His-tags
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
705980
Escherichia coli
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
Escherichia coli
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein with N-terminal 6His-tags
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
705980
Escherichia coli
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme
Escherichia coli
physiological function
both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme
Escherichia coli
physiological function
both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
Escherichia coli
Other publictions for EC 2.1.1.198
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733030
Zhao
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. F
70
1256-1259
2014
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1
1
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-
-
-
-
1
1
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1
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1
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1
1
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1
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1
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1
1
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1
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1
1
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735241
Lioy
Aminoglycoside resistance 16S ...
Escherichia coli, Escherichia coli BW25113
RNA
20
382-391
2014
-
-
-
-
-
-
-
-
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3
-
28
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3
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3
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3
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-
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-
-
-
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1
1
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-
705980
Kimura
Fine-tuning of the ribosomal d ...
Escherichia coli
Nucleic Acids Res.
38
1341-1352
2010
-
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1
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-
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-
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1
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1
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1
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1
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1
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1
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1
-
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1
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-
-
-
-
-
-
-
-
-
2
2
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