Cloned (Comment) | Organism |
---|---|
gene rlmM, expression of His-tagged RlmM in Escherichia coli strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged RlmM free or in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, 0.0015 ml 10 mg/ml protein solution os mixed with 0.0015 ml of reservoir solution containing 0.3 M ammonium tartrate, pH 7.0, and 20% PEG 3350, 20°C, 1 week, two crystal forms, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively, molecular replacement | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | Escherichia coli | RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | Escherichia coli BW25113 | RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ADR6 | gene rlmM | - |
Escherichia coli BW25113 | P0ADR6 | gene rlmM | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged RlmM from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | 23S rRNA substrate structure, overview. The S-adenosyl-L-methionine-binding site is open and shallow, suggesting that RNA substrate binding may be required to form a conformation needed for catalysis. RlmM uses one side of the two domains and the inter-domain linker to recognize its RNA substrate | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain | Escherichia coli BW25113 | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | 23S rRNA substrate structure, overview. The S-adenosyl-L-methionine-binding site is open and shallow, suggesting that RNA substrate binding may be required to form a conformation needed for catalysis. RlmM uses one side of the two domains and the inter-domain linker to recognize its RNA substrate | Escherichia coli BW25113 | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | RlmM consists of an N-terminal THUMP domain and a C-terminal catalytic Rossmann-like fold MTase domain in a distinct arrangement, detailed structure analysis, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2O-ribose methyltransferase | - |
Escherichia coli |
RlmM | - |
Escherichia coli |
ygdE | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Na2SO4 gives the highest thermal stability to RlmM | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | the S-adenosyl-L-methionine-binding site is open and shallow | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the catalytic domain of RlmM is closely related to YiiB, TlyA and fibrillarins, with the second K of the catalytic tetrad KDKE shifted by two residues at the C-terminal end of a beta strand compared with most 2'O MTases | Escherichia coli |
physiological function | RlmM catalyzes the S-adenosyl methionine-dependent 20O methylation of C2498 in 23S ribosomal RNA of Escherichia coli | Escherichia coli |