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Literature summary for 2.1.1.186 extracted from

  • Purta, E.; O'Connor, M.; Bujnicki, J.M.; Douthwaite, S.
    YgdE is the 2'-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA (2009), Mol. Microbiol., 72, 1147-1158.
    View publication on PubMed
Search for more literature for 2.1.1.186

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA Escherichia coli YgdE is the 2'-O-methyltransferase responsible for modifying 23S rRNA nucleotide C2498. Modification occurs before assembly of the 50S ribosomal subunit S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ADR6
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA YgdE is the 2'-O-methyltransferase responsible for modifying 23S rRNA nucleotide C2498. Modification occurs before assembly of the 50S ribosomal subunit Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA the recombinant YgdE methyltransferase modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes. Nucleotide C2498 is situated within a highly conserved and heavily modified rRNA sequence, and the activity of YgdE is influenced by other modification enzymes that target this region Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA
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 ?

Synonyms

Synonyms Comment Organism
rRNA large subunit methyltransferase M
-
 Escherichia coli
ygdE
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction C2498 methylation and the putative second methylation observed in the wild-type (YgdE+) strain are completely absent in the DygdE strain. Inactivation of the ygdE gene leads to loss of methylation at nucleotide C2498. The loss of ygdE function causes a slight reduction in bacterial fitness Escherichia coli