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Literature summary for 2.1.1.184 extracted from

  • Denoya, C.; Dubnau, D.
    Mono- and dimethylating activities and kinetic studies of the ermC 23 S rRNA methyltransferase (1989), J. Biol. Chem., 264, 2615-2624.
    View publication on PubMed
Search for more literature for 2.1.1.184

Inhibitors

Inhibitors Comment Organism Structure
N6-dimethyladenine2085 containing 23S rRNA linear competitive inhibition kinetics with RNA as the variable substrate, mixed inhibition with S-adenosyl-L-methionine as the variable substrate Bacillus subtilis
S-adenosyl-L-homocysteine linear competitive pattern with S-adenosyl-L-methionine as the variable substrate, and a mixed inhibition kinetics with RNA Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000354
-
 S-adenosyl-L-methionine pH 7.5, 37°C Bacillus subtilis
0.012
-
 adenine2085 in 23S rRNA pH 7.5, 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA Bacillus subtilis
-
 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA Bacillus subtilis BD170
-
 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P13956 carrying plasmid pE194-cop-1 with the ermC gene
-
Bacillus subtilis BD170 P13956 carrying plasmid pE194-cop-1 with the ermC gene
-

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA = 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA ermC methylase reaction involves a sequential mechanism occurring by two consecutive random bi bi reactions Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA
-
 Bacillus subtilis 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA ermC methyltransferase produces both N6-mono and N6,N6-dimethylated adenine residues in Bacillus subtilis 23 S rRNA during the course of the reaction in vitro. The addition of the two methyl groups to each 23 S rRNA molecule takes place through a monomethylated intermediate and suggest that the enzyme dissociates from its RNA substrate between the two consecutive methylation reactions. The enzyme is able to utilize monomethylated RNA as substrate for the addition of a second methyl group with an efficiency approximately comparable to that obtained when unmethylated RNA is the initial substrate Bacillus subtilis 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA
-
 Bacillus subtilis BD170 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA ermC methyltransferase produces both N6-mono and N6,N6-dimethylated adenine residues in Bacillus subtilis 23 S rRNA during the course of the reaction in vitro. The addition of the two methyl groups to each 23 S rRNA molecule takes place through a monomethylated intermediate and suggest that the enzyme dissociates from its RNA substrate between the two consecutive methylation reactions. The enzyme is able to utilize monomethylated RNA as substrate for the addition of a second methyl group with an efficiency approximately comparable to that obtained when unmethylated RNA is the initial substrate Bacillus subtilis BD170 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
ermC 23 S rRNA methyltransferase
-
 Bacillus subtilis
ermC methylase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
 assay at Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000128
-
 N6-dimethyladenine2085 containing 23S rRNA pH 7.5, 37°C, S-adenosyl-L-methionine as the variable substrate Bacillus subtilis
0.00036
-
 N6-dimethyladenine2085 containing 23S rRNA pH 7.5, 37°C, with RNA as the variable substrate Bacillus subtilis
0.069
-
 S-adenosyl-L-homocysteine pH 7.5, 37°C, with RNA as the variable substrate Bacillus subtilis
0.086
-
 S-adenosyl-L-homocysteine pH 7.5, 37°C, S-adenosyl-L-methionine as the variable substrate Bacillus subtilis