Literature summary for 2.1.1.183 extracted from

Pulicherla, N.; Pogorzala, L.A.; Xu, Z.; O'Farrell, H.C.; Musayev, F.N.; Scarsdale, J.N.; Sia, E.A.; Culver, G.M.; Rife, J.P.
Structural and functional divergence within the Dim1/KsgA family of rRNA methyltransferases (2009), J. Mol. Biol., 391, 884-893.

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Cloned(Commentary)

Cloned (Comment)	Organism
to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1	Saccharomyces cerevisiae

Cloned (Comment)

Organism

to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1

Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E85A	the altered protein is able to complement for Dim1 but yields a protein with no catalytic activity as determined by primer extension of 18S rRNA. The expected primer extension stops at 1779 and 1780 are missing in rRNA extracted from the strain expressing catalytically inactive ScDim1. The single mutant shows no change in growth rate of yeast at 18, 25, 30, and 37°C	Saccharomyces cerevisiae
additional information	to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1, suggesting that eukaryotic-specific Dim1 function resides in the N-terminal domain, where few structural differences are observed between members of the KsgA/Dim1 family	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
processome	Dim1 in eukaryotic organisms is an essential member of the processome, a multifactor assembly that does not exist in bacteria	Saccharomyces cerevisiae	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA	Saccharomyces cerevisiae	neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation	4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	C7GSG6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4 S-adenosyl-L-methionine + adenine1779/1780 in 18S rRNA	-	Saccharomyces cerevisiae	4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA	-	?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA	neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation	Saccharomyces cerevisiae	4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
ScDim1	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation	Saccharomyces cerevisiae