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Literature summary for 2.1.1.182 extracted from

  • Demirci, H.; Belardinelli, R.; Seri, E.; Gregory, S.T.; Gualerzi, C.; Dahlberg, A.E.; Jogl, G.
    Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine (2009), J. Mol. Biol., 388, 271-282.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
full-length ksgA gene is cloned into the expression vector pET26b and overexpressed in Escherichia coli strain BL21Star Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch technique under oil at 4°C, crystal structures of KsgA in the apo form and with 5’-methylthioadenosine or adenosine bound in the cofactor-binding site Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SM60
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-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SM60
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-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
?
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
?

Synonyms

Synonyms Comment Organism
KsgA
-
Thermus thermophilus

General Information

General Information Comment Organism
malfunction loss of this dimethylation confers resistance to the antibiotic kasugamycin Thermus thermophilus