Cloned (Comment) | Organism |
---|---|
full-length ksgA gene is cloned into the expression vector pET26b and overexpressed in Escherichia coli strain BL21Star | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
microbatch technique under oil at 4°C, crystal structures of KsgA in the apo form and with 5-methylthioadenosine or adenosine bound in the cofactor-binding site | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SM60 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SM60 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA | KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate | Thermus thermophilus | 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA | - |
? | |
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA | KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KsgA | - |
Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of this dimethylation confers resistance to the antibiotic kasugamycin | Thermus thermophilus |