BRENDA - Enzyme Database show
show all sequences of 2.1.1.182

Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions

Poldermans, B.; Roza, L.; van Knippenberg, P.H.; J. Biol. Chem. 254, 9094-9100 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
a mixture of 30 S ribosomal proteins inhibits methylation of mutant 30 S ribosomes. This inhibition can be ascribed to ribosomal protein S21. Initiation factor 3 partially inhibits methylation of mutant 30 S ribosomes
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
2-3 mM, required
Escherichia coli
additional information
K+ and NH4+ in the range of 10 to 150 mM have little effect on methylation
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA
-
704371
Escherichia coli
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 30000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.8
7.8
change of pH from 7.8 to 6.8 reduces methylation by 50%
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
a mixture of 30 S ribosomal proteins inhibits methylation of mutant 30 S ribosomes. This inhibition can be ascribed to ribosomal protein S21. Initiation factor 3 partially inhibits methylation of mutant 30 S ribosomes
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
2-3 mM, required
Escherichia coli
additional information
K+ and NH4+ in the range of 10 to 150 mM have little effect on methylation
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA
-
704371
Escherichia coli
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 30000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.8
7.8
change of pH from 7.8 to 6.8 reduces methylation by 50%
Escherichia coli
Other publictions for EC 2.1.1.182
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733426
Kyuma
16S rRNA methyltransferase Ksg ...
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Biochimie
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166-174
2015
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2
2
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733980
Phunpruch
A role for 16S rRNA dimethyltr ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Antimicrob. Agents
41
548-551
2013
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2
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1
1
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718932
O'Farrell
Control of substrate specifici ...
Escherichia coli
Biochemistry
51
466-474
2012
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1
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3
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2
-
1
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1
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2
2
-
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701730
Ochi
Inactivation of KsgA, a 16S rR ...
Bacillus subtilis
Antimicrob. Agents Chemother.
53
193-201
2009
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1
1
-
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702903
Binet
The chlamydial functional homo ...
Chlamydia psittaci, Chlamydia psittaci 6BC, Chlamydia trachomatis, Chlamydia trachomatis L2
BMC Microbiol.
9
0000
2009
-
1
2
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4
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2
2
-
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704036
Duffin
ksgA mutations confer resistan ...
Neisseria gonorrhoeae
Int. J. Antimicrob. Agents
33
321-327
2009
-
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1
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1
1
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705141
Demirci
Structural rearrangements in t ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
J. Mol. Biol.
388
271-282
2009
-
-
1
1
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1
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2
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1
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2
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2
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1
1
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705968
Zhang-Akiyama
KsgA, a 16S rRNA adenine methy ...
Escherichia coli
Nucleic Acids Res.
37
2116-2125
2009
-
-
1
-
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-
-
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1
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1
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1
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1
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1
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1
1
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706787
Tu
Structural basis for binding o ...
Aquifex aeolicus
Structure
17
374-385
2009
-
-
1
1
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2
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1
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705774
Connolly
Mechanistic insight into the r ...
Escherichia coli
Mol. Microbiol.
70
1062-1075
2008
-
1
1
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1
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2
2
-
-
-
704274
Inoue
Dissection of 16S rRNA methylt ...
Escherichia coli
J. Bacteriol.
189
8510-8518
2007
-
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4
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1
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1
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1
1
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701901
Desai
The adenosine dimethyltransfer ...
Escherichia coli
Arch. Biochem. Biophys.
449
57-63
2006
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1
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1
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706715
O'Farrell
Recognition of a complex subst ...
Escherichia coli
RNA
12
725-733
2006
-
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1
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1
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1
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662620
O'Farrell
Crystal structure of KsgA, a u ...
Escherichia coli
J. Mol. Biol.
339
337-353
2004
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1
1
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485442
Formenoy
Methylation of the conserved A ...
Escherichia coli
Biochimie
76
1123-1128
1994
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705108
Lafontaine
The DIM1 gene responsible for ...
Escherichia coli
J. Mol. Biol.
241
492-497
1994
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702395
Cunningham
Site-specific mutation of the ...
Escherichia coli
Biochim. Biophys. Acta
1050
18-26
1990
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703844
van Buul
Nucleotide sequence of the ksg ...
Escherichia coli
Gene
38
65-72
1985
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705740
Andresson
Some properties of the ribosom ...
Escherichia coli
Mol. Gen. Genet.
179
217-222
1980
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3
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704371
Poldermans
Studies on the function of two ...
Escherichia coli
J. Biol. Chem.
254
9094-9100
1979
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1
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2
1
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1
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1
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1
1
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705860
Helser
Mechanism of kasugamycin resis ...
Escherichia coli
Nat. New Biol.
235
6-9
1972
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1
1
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705859
Helser
Change in methylation of 16S r ...
Escherichia coli
Nat. New Biol.
233
12-14
1971
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