Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | a mixture of 30 S ribosomal proteins inhibits methylation of mutant 30 S ribosomes. This inhibition can be ascribed to ribosomal protein S21. Initiation factor 3 partially inhibits methylation of mutant 30 S ribosomes | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2-3 mM, required | Escherichia coli | |
additional information | K+ and NH4+ in the range of 10 to 150 mM have little effect on methylation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA | - |
Escherichia coli | 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.8 | change of pH from 7.8 to 6.8 reduces methylation by 50% | Escherichia coli |