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Literature summary for 2.1.1.179 extracted from

  • Correa, L.L.; Witek, M.A.; Zelinskaya, N.; Picao, R.C.; Conn, G.L.
    Heterologous expression and functional characterization of the exogenously acquired aminoglycoside resistance methyltransferases RmtD, RmtD2, and RmtG (2015), Antimicrob. Agents Chemother., 60, 699-702.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.179

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
 x * 29500, RmtD, SDS-PAGE Escherichia coli
29500
-
 x * 29500, RmtD2, SDS-PAGE Escherichia coli
31500
-
 x * 31500, RmtG, SDS-PAGE Escherichia coli
32400
-
 x * 32400, SDS-PAGE Micromonospora zionensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA Micromonospora zionensis
-
 S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Micromonospora zionensis Q7M0R2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and Superdex 75 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
 Micromonospora zionensis S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 32400, SDS-PAGE Micromonospora zionensis
? x * 29500, RmtD, SDS-PAGE Escherichia coli
? x * 29500, RmtD2, SDS-PAGE Escherichia coli
? x * 31500, RmtG, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
16S rRNA (m7G1405) methyltransferase
-
 Escherichia coli
16S rRNA (m7G1405) methyltransferase
-
 Micromonospora zionensis
RmtD
-
 Escherichia coli
RmtD2
-
 Escherichia coli
RmtG
-
 Escherichia coli
sgm
-
 Micromonospora zionensis