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show all sequences of 2.1.1.179

Exogenously acquired 16S rRNA methyltransferases found in aminoglycoside-resistant pathogenic Gram-negative bacteria: an update

Wachino, J.; Arakawa, Y.; Drug Resist. Updat. 15, 133-148 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Citrobacter freundii
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora echinospora
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora inyonensis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora olivasterospora
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora rosea
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora zionensis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Proteus mirabilis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria; development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Pseudomonas aeruginosa
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Serratia marcescens
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Streptoalloteichus hindustanus
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Streptomyces kanamyceticus
Cloned(Commentary)
Commentary
Organism
gene fmrO, phylogenetic analysis, sequence comparisons
Micromonospora olivasterospora
gene grmA, phylogenetic analysis, sequence comparisons
Micromonospora echinospora
gene grmB, phylogenetic analysis, sequence comparisons
Micromonospora rosea
gene kmr, phylogenetic analysis, sequence comparisons
Streptomyces kanamyceticus
gene nbrB, phylogenetic analysis, sequence comparisons
Streptoalloteichus hindustanus
gene rmtA, phylogenetic analysis, sequence comparisons; phylogenetic analysis, sequence comparisons
Pseudomonas aeruginosa
gene rmtB, phylogenetic analysis, sequence comparisons
Serratia marcescens
gene rmtC, phylogenetic analysis, sequence comparisons
Proteus mirabilis
gene rmtD2, phylogenetic analysis, sequence comparisons
Citrobacter freundii
gene sgm, phylogenetic analysis, sequence comparisons
Micromonospora zionensis
gene smr1, phylogenetic analysis, sequence comparisons
Micromonospora inyonensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora zionensis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora echinospora
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Proteus mirabilis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora inyonensis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Streptoalloteichus hindustanus
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Streptomyces kanamyceticus
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Serratia marcescens
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Pseudomonas aeruginosa
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Citrobacter freundii
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora olivasterospora
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora rosea
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Proteus mirabilis ARS68
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrobacter freundii
E9N3S1
RmtD2; gene rmtD2
-
Micromonospora echinospora
Q70KC8
GrmA; gene grmA
-
Micromonospora inyonensis
Q5Y813
Smr1; gene smr1
-
Micromonospora olivasterospora
Q08325
FmrO; gene fmrO
-
Micromonospora rosea
P24619
GrmB; gene grmB
-
Micromonospora zionensis
Q7M0R2
Sgm; gene sgm
-
Proteus mirabilis
Q33DX5
RmtC; gene rmtC
-
Proteus mirabilis ARS68
Q33DX5
RmtC; gene rmtC
-
Pseudomonas aeruginosa
A0MK31
RmtD; gene rmtD
-
Pseudomonas aeruginosa
Q8GRA1
RmtA; gene rmtA
-
Serratia marcescens
Q76G15
RmtB; gene rmtB
-
Streptoalloteichus hindustanus
O52472
NbrB; gene nbrB
-
Streptomyces kanamyceticus
Q75PS4
Kmr; gene kmr
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora zionensis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora echinospora
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Proteus mirabilis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora inyonensis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Streptoalloteichus hindustanus
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Streptomyces kanamyceticus
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Serratia marcescens
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Pseudomonas aeruginosa
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Citrobacter freundii
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora olivasterospora
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora rosea
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Proteus mirabilis ARS68
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Citrobacter freundii
S-adenosyl-L-methionine
-
Micromonospora echinospora
S-adenosyl-L-methionine
-
Micromonospora inyonensis
S-adenosyl-L-methionine
-
Micromonospora olivasterospora
S-adenosyl-L-methionine
-
Micromonospora rosea
S-adenosyl-L-methionine
-
Micromonospora zionensis
S-adenosyl-L-methionine
-
Proteus mirabilis
S-adenosyl-L-methionine
;
Pseudomonas aeruginosa
S-adenosyl-L-methionine
-
Serratia marcescens
S-adenosyl-L-methionine
-
Streptoalloteichus hindustanus
S-adenosyl-L-methionine
-
Streptomyces kanamyceticus
Application (protein specific)
Application
Commentary
Organism
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Citrobacter freundii
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora echinospora
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora inyonensis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora olivasterospora
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora rosea
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Micromonospora zionensis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Proteus mirabilis
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Pseudomonas aeruginosa
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Serratia marcescens
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Streptoalloteichus hindustanus
drug development
development of potent agents against 16S-RMTase producers as targets for treatment of multidrug resistant bacteria
Streptomyces kanamyceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene fmrO, phylogenetic analysis, sequence comparisons
Micromonospora olivasterospora
gene grmA, phylogenetic analysis, sequence comparisons
Micromonospora echinospora
gene grmB, phylogenetic analysis, sequence comparisons
Micromonospora rosea
gene kmr, phylogenetic analysis, sequence comparisons
Streptomyces kanamyceticus
gene nbrB, phylogenetic analysis, sequence comparisons
Streptoalloteichus hindustanus
gene rmtA, phylogenetic analysis, sequence comparisons
Pseudomonas aeruginosa
gene rmtB, phylogenetic analysis, sequence comparisons
Serratia marcescens
gene rmtC, phylogenetic analysis, sequence comparisons
Proteus mirabilis
gene rmtD2, phylogenetic analysis, sequence comparisons
Citrobacter freundii
gene sgm, phylogenetic analysis, sequence comparisons
Micromonospora zionensis
gene smr1, phylogenetic analysis, sequence comparisons
Micromonospora inyonensis
phylogenetic analysis, sequence comparisons
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Citrobacter freundii
S-adenosyl-L-methionine
-
Micromonospora echinospora
S-adenosyl-L-methionine
-
Micromonospora inyonensis
S-adenosyl-L-methionine
-
Micromonospora olivasterospora
S-adenosyl-L-methionine
-
Micromonospora rosea
S-adenosyl-L-methionine
-
Micromonospora zionensis
S-adenosyl-L-methionine
-
Proteus mirabilis
S-adenosyl-L-methionine
-
Pseudomonas aeruginosa
S-adenosyl-L-methionine
-
Serratia marcescens
S-adenosyl-L-methionine
-
Streptoalloteichus hindustanus
S-adenosyl-L-methionine
-
Streptomyces kanamyceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora zionensis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora echinospora
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Proteus mirabilis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora inyonensis
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Streptoalloteichus hindustanus
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Streptomyces kanamyceticus
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Serratia marcescens
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Pseudomonas aeruginosa
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Citrobacter freundii
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora olivasterospora
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Micromonospora rosea
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
Proteus mirabilis ARS68
-
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora zionensis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora echinospora
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Proteus mirabilis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora inyonensis
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Streptoalloteichus hindustanus
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Streptomyces kanamyceticus
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Serratia marcescens
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Pseudomonas aeruginosa
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Citrobacter freundii
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora olivasterospora
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Micromonospora rosea
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
719353
Proteus mirabilis ARS68
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
RmtD2 shows aquired N7-G1405 16S-RMTase activity
Citrobacter freundii
evolution
FmrO shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora olivasterospora
evolution
RmtC shows aquired N7-G1405 16S-RMTase activity
Proteus mirabilis
evolution
RmtA shows aquired N7-G1405 16S-RMTase activity; RmtD shows aquired N7-G1405 16S-RMTase activity
Pseudomonas aeruginosa
evolution
RmtB shows aquired N7-G1405 16S-RMTase activity
Serratia marcescens
additional information
GrmA shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora echinospora
additional information
Smr1 shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora inyonensis
additional information
GrmB shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora rosea
additional information
Sgm shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora zionensis
additional information
NbrB shows intrinsic N7-G1405 16S-RMTase activity
Streptoalloteichus hindustanus
additional information
Kmr shows intrinsic N7-G1405 16S-RMTase activity
Streptomyces kanamyceticus
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Citrobacter freundii
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora echinospora
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora inyonensis
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis, These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora olivasterospora
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora rosea
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora zionensis
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Proteus mirabilis
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Pseudomonas aeruginosa clinical isolates show high-level resistance to clinically useful aminoglycosides through the production of acquired 16S-RMTase. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview; the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Pseudomonas aeruginosa clinical isolates show high-level resistance to clinically useful aminoglycosides through the production of acquired 16S-RMTase. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Pseudomonas aeruginosa
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Serratia marcescens
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Streptoalloteichus hindustanus
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Streptomyces kanamyceticus
General Information (protein specific)
General Information
Commentary
Organism
evolution
RmtD2 shows aquired N7-G1405 16S-RMTase activity
Citrobacter freundii
evolution
FmrO shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora olivasterospora
evolution
RmtC shows aquired N7-G1405 16S-RMTase activity
Proteus mirabilis
evolution
RmtA shows aquired N7-G1405 16S-RMTase activity
Pseudomonas aeruginosa
evolution
RmtD shows aquired N7-G1405 16S-RMTase activity
Pseudomonas aeruginosa
evolution
RmtB shows aquired N7-G1405 16S-RMTase activity
Serratia marcescens
additional information
GrmA shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora echinospora
additional information
Smr1 shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora inyonensis
additional information
GrmB shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora rosea
additional information
Sgm shows intrinsic N7-G1405 16S-RMTase activity
Micromonospora zionensis
additional information
NbrB shows intrinsic N7-G1405 16S-RMTase activity
Streptoalloteichus hindustanus
additional information
Kmr shows intrinsic N7-G1405 16S-RMTase activity
Streptomyces kanamyceticus
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Citrobacter freundii
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora echinospora
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora inyonensis
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis, These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora olivasterospora
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora rosea
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Micromonospora zionensis
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Proteus mirabilis
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Pseudomonas aeruginosa clinical isolates show high-level resistance to clinically useful aminoglycosides through the production of acquired 16S-RMTase. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Pseudomonas aeruginosa
physiological function
the enzyme adds the methyl group of S-adenosyl-L-methionine to the specific nucleotides at the A-site of 16S rRNA, which interferes with aminoglycoside binding to the target. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Serratia marcescens
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Streptoalloteichus hindustanus
physiological function
most aminoglycosides bind to the decoding aminoacyl-tRNA recognition site (A-site) of the 16S rRNA that composes bacterial 30S ribosome, and subsequently interfere with bacterial growth through blocking of protein synthesis. These aminoglycoside-producing actinomycetes are inherently resistant to aminoglycosides, because they harbor intrinsic 16S rRNA methyltransferase genes, that can confer aminoglycoside resistance to bacteria by modifying specific nucleotide residues in the aminoglycoside binding site of 16S rRNA. Aminoglycoside resistance profile provided by N7-G1405 16S-RMTases, overview
Streptomyces kanamyceticus
Other publictions for EC 2.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733120
Correa
Heterologous expression and fu ...
Escherichia coli, Micromonospora zionensis
Antimicrob. Agents Chemother.
60
699-702
2015
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735241
Lioy
Aminoglycoside resistance 16S ...
Escherichia coli, Escherichia coli MM294
RNA
20
382-391
2014
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735238
Gutierrez
Indigenous and acquired modifi ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa BB1285
RNA Biol.
10
1324-1332
2013
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718596
Gutierrez
Fitness cost and interference ...
Escherichia coli, Escherichia coli BW25113
Antimicrob. Agents Chemother.
56
2335-2341
2012
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31
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2
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718603
Galimand
RmtF, a new member of the amin ...
Klebsiella pneumoniae, Klebsiella pneumoniae BM4686
Antimicrob. Agents Chemother.
56
3960-3962
2012
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719353
Wachino
Exogenously acquired 16S rRNA ...
Citrobacter freundii, Micromonospora echinospora, Micromonospora inyonensis, Micromonospora olivasterospora, Micromonospora rosea, Micromonospora zionensis, Proteus mirabilis, Proteus mirabilis ARS68, Pseudomonas aeruginosa, Serratia marcescens, Streptoalloteichus hindustanus, Streptomyces kanamyceticus
Drug Resist. Updat.
15
133-148
2012
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11
11
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13
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21
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22
24
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705988
Husain
Structural basis for the methy ...
Micromonospora zionensis
Nucleic Acids Res.
38
4120-4132
2010
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1
1
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4
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712038
Wachino
RmtC introduces G1405 methylat ...
Proteus mirabilis, Proteus mirabilis ARS68
FEMS Microbiol. Lett.
311
56-60
2010
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1
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705972
Savic
Determination of the target nu ...
Frankia sp., Frankia sp. CcI3, Micromonospora echinospora, Micromonospora zionensis
Nucleic Acids Res.
37
5420-5431
2009
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706724
Cubrilo
The aminoglycoside resistance ...
Micromonospora zionensis
RNA
15
1492-1497
2009
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712741
Schmitt
Structural bases for 16 S rRNA ...
Escherichia coli
J. Mol. Biol.
388
570-582
2009
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1
1
10
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702415
Maravic Vlahovicek
Modeling and experimental anal ...
Micromonospora zionensis
Biochim. Biophys. Acta
1784
582-590
2008
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1
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14
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14
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704280
Savic
Critical residues for cofactor ...
Micromonospora zionensis
J. Bacteriol.
190
5855-5861
2008
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1
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7
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706701
Tomic
Aminoglycoside resistance gene ...
Micromonospora zionensis
Res. Microbiol.
159
658-662
2008
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710881
Périchon
Transferable resistance to ami ...
plasmid pIP1206
Antimicrob. Agents Chemother.
51
2464-2469
2007
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1
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710880
Wachino
Novel plasmid-mediated 16S rRN ...
Proteus mirabilis, Proteus mirabilis ARS68
Antimicrob. Agents Chemother.
50
178-184
2006
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712738
Liou
Aminoglycoside resistance by A ...
plasmid pAT780
J. Mol. Biol.
359
358-364
2006
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704254
Kojic
Translational autoregulation o ...
Micromonospora zionensis
J. Bacteriol.
178
5493-5498
1996
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704248
Kojic
Cloning and characterization o ...
Micromonospora zionensis
J. Bacteriol.
174
7868-7872
1992
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705105
Beauclerk
Sites of action of two ribosom ...
Micromonospora echinospora
J. Mol. Biol.
193
661-671
1987
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