Cloned (Comment) | Organism |
---|---|
gene rmtC, expression of wild-type enzyme in Escherichi coli Top10 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview | ? | - |
? | |
additional information | Escherichia coli BW25113 | RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview | ? | - |
? | |
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA | Escherichia coli BW25113 | - |
S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
i.e. strain BB1074, gene rmtC | - |
Escherichia coli BW25113 | - |
i.e. strain BB1074, gene rmtC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview | Escherichia coli | ? | - |
? | |
additional information | RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview | Escherichia coli BW25113 | ? | - |
? | |
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1405 in 16S rRNA | - |
Escherichia coli BW25113 | S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methyltransferase RmtC | - |
Escherichia coli |
RmtC | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | acquisition of rmtC does not entail a fitness cost for the bacterium | Escherichia coli |
physiological function | the enzyme confers high-level resistance to 4,6-disubstituted aminoglycosides through methylation of the G1405 residue in the 16S rRNA. RmtC impedes methylation by the housekeeping methyltransferase RsmF, EC 2.1.1.178, at position C1407 | Escherichia coli |