BRENDA - Enzyme Database show
show all sequences of 2.1.1.179

Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases

Husain, N.; Tkaczuk, K.L.; Tulsidas, S.R.; Kaminska, K.H.; Cubrilo, S.; Maravic-Vlahovicek, G.; Bujnicki, J.M.; Sivaraman, J.; Nucleic Acids Res. 38, 4120-4132 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression
Micromonospora zionensis
Crystallization (Commentary)
Crystallization
Organism
purified Sgm is complexed with 5 mM of the cofactors S-adenosylmethionine/S-adenosylhomocysteine. Crystallization trials are carried out at room temperature by hanging-drop vapor-diffusion method, structure of Sgm in complex with cofactors S-adenosylmethionine and S-adenosylhomocysteine is determined at 2.0 A and 2.1 A resolution, respectively
Micromonospora zionensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
Micromonospora zionensis
the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micromonospora zionensis
Q7M0R2
-
-
Purification (Commentary)
Commentary
Organism
-
Micromonospora zionensis
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA
the mechanism of methylation of G1405 by Sgm is proposed and compared with other m7G methyltransferases
Micromonospora zionensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
705988
Micromonospora zionensis
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA
705988
Micromonospora zionensis
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression
Micromonospora zionensis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified Sgm is complexed with 5 mM of the cofactors S-adenosylmethionine/S-adenosylhomocysteine. Crystallization trials are carried out at room temperature by hanging-drop vapor-diffusion method, structure of Sgm in complex with cofactors S-adenosylmethionine and S-adenosylhomocysteine is determined at 2.0 A and 2.1 A resolution, respectively
Micromonospora zionensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
Micromonospora zionensis
the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Micromonospora zionensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
705988
Micromonospora zionensis
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA
705988
Micromonospora zionensis
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
Other publictions for EC 2.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733120
Correa
Heterologous expression and fu ...
Escherichia coli, Micromonospora zionensis
Antimicrob. Agents Chemother.
60
699-702
2015
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1
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-
-
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3
2
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3
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1
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2
2
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1
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3
2
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1
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2
2
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-
-
-
-
-
-
-
-
-
735241
Lioy
Aminoglycoside resistance 16S ...
Escherichia coli, Escherichia coli MM294
RNA
20
382-391
2014
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
-
-
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3
-
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1
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3
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3
-
-
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-
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-
735238
Gutierrez
Indigenous and acquired modifi ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa BB1285
RNA Biol.
10
1324-1332
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
2
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2
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2
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2
-
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-
-
-
-
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-
-
-
1
1
-
-
-
718596
Gutierrez
Fitness cost and interference ...
Escherichia coli, Escherichia coli BW25113
Antimicrob. Agents Chemother.
56
2335-2341
2012
-
-
1
-
1
-
-
-
-
-
-
4
-
31
-
-
-
-
-
-
-
-
4
-
-
-
-
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-
-
-
1
-
-
-
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1
1
-
1
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-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
718603
Galimand
RmtF, a new member of the amin ...
Klebsiella pneumoniae, Klebsiella pneumoniae BM4686
Antimicrob. Agents Chemother.
56
3960-3962
2012
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
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2
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1
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1
1
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2
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-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
719353
Wachino
Exogenously acquired 16S rRNA ...
Citrobacter freundii, Micromonospora echinospora, Micromonospora inyonensis, Micromonospora olivasterospora, Micromonospora rosea, Micromonospora zionensis, Proteus mirabilis, Proteus mirabilis ARS68, Pseudomonas aeruginosa, Serratia marcescens, Streptoalloteichus hindustanus, Streptomyces kanamyceticus
Drug Resist. Updat.
15
133-148
2012
-
11
11
-
-
-
-
-
-
-
-
13
-
21
-
-
-
-
-
-
-
-
13
-
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-
-
11
-
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-
12
12
12
-
-
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-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
22
24
-
-
-
705988
Husain
Structural basis for the methy ...
Micromonospora zionensis
Nucleic Acids Res.
38
4120-4132
2010
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
1
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2
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1
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1
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1
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1
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-
-
2
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
712038
Wachino
RmtC introduces G1405 methylat ...
Proteus mirabilis, Proteus mirabilis ARS68
FEMS Microbiol. Lett.
311
56-60
2010
-
-
1
-
-
-
-
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2
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4
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1
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4
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1
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2
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1
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4
-
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-
-
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-
705972
Savic
Determination of the target nu ...
Frankia sp., Frankia sp. CcI3, Micromonospora echinospora, Micromonospora zionensis
Nucleic Acids Res.
37
5420-5431
2009
-
-
-
-
-
-
-
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4
-
4
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8
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4
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8
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706724
Cubrilo
The aminoglycoside resistance ...
Micromonospora zionensis
RNA
15
1492-1497
2009
-
-
1
-
-
-
-
-
-
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1
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2
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1
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1
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1
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1
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-
712741
Schmitt
Structural bases for 16 S rRNA ...
Escherichia coli
J. Mol. Biol.
388
570-582
2009
-
-
1
1
10
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
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2
-
1
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1
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1
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1
10
-
-
-
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1
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1
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2
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1
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1
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-
702415
Maravic Vlahovicek
Modeling and experimental anal ...
Micromonospora zionensis
Biochim. Biophys. Acta
1784
582-590
2008
-
-
1
-
14
-
-
-
-
-
-
-
-
2
-
-
1
1
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-
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-
1
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1
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14
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1
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-
1
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-
704280
Savic
Critical residues for cofactor ...
Micromonospora zionensis
J. Bacteriol.
190
5855-5861
2008
-
-
1
-
7
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
-
1
-
1
-
1
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1
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7
-
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-
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-
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-
-
1
-
1
-
1
-
-
-
-
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-
-
-
-
706701
Tomic
Aminoglycoside resistance gene ...
Micromonospora zionensis
Res. Microbiol.
159
658-662
2008
-
-
1
-
-
-
-
-
-
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1
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1
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1
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1
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-
710881
Périchon
Transferable resistance to ami ...
plasmid pIP1206
Antimicrob. Agents Chemother.
51
2464-2469
2007
-
-
1
-
-
-
-
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-
1
-
1
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1
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1
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1
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1
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-
710880
Wachino
Novel plasmid-mediated 16S rRN ...
Proteus mirabilis, Proteus mirabilis ARS68
Antimicrob. Agents Chemother.
50
178-184
2006
-
-
1
-
-
-
-
-
-
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4
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1
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1
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1
1
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712738
Liou
Aminoglycoside resistance by A ...
plasmid pAT780
J. Mol. Biol.
359
358-364
2006
-
-
-
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1
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1
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1
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2
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1
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1
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1
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1
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2
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1
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1
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1
1
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704254
Kojic
Translational autoregulation o ...
Micromonospora zionensis
J. Bacteriol.
178
5493-5498
1996
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1
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3
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1
1
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704248
Kojic
Cloning and characterization o ...
Micromonospora zionensis
J. Bacteriol.
174
7868-7872
1992
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1
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1
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5
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1
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1
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705105
Beauclerk
Sites of action of two ribosom ...
Micromonospora echinospora
J. Mol. Biol.
193
661-671
1987
-
-
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1
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1
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1
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