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Literature summary for 2.1.1.178 extracted from

  • Gutierrez, B.; Escudero, J.A.; San Millan, A.; Hidalgo, L.; Carrilero, L.; Ovejero, C.M.; Santos-Lopez, A.; Thomas-Lopez, D.; Gonzalez-Zorn, B.
    Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases (2012), Antimicrob. Agents Chemother., 56, 2335-2341.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene rsmF, expression of wild-type enzyme in Escherichi coli Top10 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information RmtC, EC 2.1.1.179, in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Escherichia coli nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Escherichia coli BW25113 nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
i.e. strain BB1074, gene rsmF
-
Escherichia coli BW25113
-
i.e. strain BB1074, gene rsmF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
?

Synonyms

Synonyms Comment Organism
housekeeping methyltransferase RsmF
-
Escherichia coli
RsmF housekeeping methyltransferase
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Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli

General Information

General Information Comment Organism
malfunction DELTArsmF null mutant bacteria have a decreased susceptibility to aminoglycosides, including 4,6- and 4,5-deoxystreptamine aminoglycosides Escherichia coli
physiological function the housekeeping methylation at C1407 is involved in intrinsic aminoglycoside susceptibility in Escherichia coli Escherichia coli