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Literature summary for 2.1.1.178 extracted from
- YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407 (2006), J. Mol. Biol., 359, 777-786.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the yebU gene is cloned as a partial and as a fulllength sequence with the respective purposes of creating a knockout strain and expressing recombinant YebU protein. Full-length YebU protein with a C-terminal histidine-tag is expressed in Escherichia coli | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | Escherichia coli | nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P76273 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YebU | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inactivation of the yebU gene leads to loss of methylation at C1407 in 16S rRNA. yebU knockout strains display slower growth and reduced fitness in competition with wild-type cells (at 37°C and 42°C) | Escherichia coli |
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Release 2023.1 (January 2023)
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