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Literature summary for 2.1.1.174 extracted from

  • Sergiev, P.V.; Lesnyak, D.V.; Bogdanov, A.A.; Dontsova, O.A.
    Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA (2006), J. Mol. Biol., 364, 26-31.
    View publication on PubMed
Search for more literature for 2.1.1.174

Cloned(Commentary)

Cloned (Comment) Organism
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine1835 in 23S rRNA Escherichia coli the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2 S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q0T0I4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine1835 in 23S rRNA the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA recombinant YgjO protein is able to methylate in vitro protein-free 23S rRNA, but not assembled 50S subunits purified from the ygjO knock-out strain Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ribosomal RNA large subunit methyltransferase G
-
 Escherichia coli
RlmG
-
 Escherichia coli
ygjO
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction knock-out of the ygjO gene leads to loss of modification at G1835. Lack of the G1835 methylation causes growth retardation, especially at temperatures higher than optimal and in poor media Escherichia coli