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Literature summary for 2.1.1.173 extracted from

  • Wang, K.T.; Desmolaize, B.; Nan, J.; Zhang, X.W.; Li, L.F.; Douthwaite, S.; Su, X.D.
    Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA (2012), Nucleic Acids Res., 40, 5138-5148.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.173

Protein Variants

Protein Variants Comment Organism
additional information generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY ?
-
 ?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA Escherichia coli
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 S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA Streptococcus mutans Smu472 adds the m2G2445 modification S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75864 gene ycbY
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Streptococcus mutans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY Escherichia coli ?
-
 ?
additional information Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs Streptococcus mutans ?
-
 ?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA m7G2069 modification by YcbY is stoichiometric Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
 Streptococcus mutans S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA Smu472 adds the m2G2445 modification Streptococcus mutans S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA m2G2445 modification by YcbY is stoichiometric Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
More structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview Streptococcus mutans
More structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview Escherichia coli

Synonyms

Synonyms Comment Organism
m2G2445 methyltransferase
-
 Streptococcus mutans
m2G2445 methyltransferase
-
 Escherichia coli
ribosomal large subunit methyltransferase
-
 Escherichia coli
RlmKL
-
 Escherichia coli
Smu472
-
 Streptococcus mutans
ycbY
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Streptococcus mutans
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
evolution the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered Streptococcus mutans
evolution the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered Escherichia coli
physiological function the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification Escherichia coli