Literature summary for 2.1.1.173 extracted from

Lesnyak, D.V.; Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.
Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA (2006), J. Mol. Biol., 364, 20-25.

Search for more literature for 2.1.1.173

Cloned(Commentary)

Cloned (Comment)	Organism
YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79000	-	x * 79000, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine2445 in 23S rRNA	Escherichia coli	N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75864	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine2445 in 23S rRNA	N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA	recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?

Subunits

Subunits	Comment	Organism
?	x * 79000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
RlmL	-	Escherichia coli
ycbY	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	knock-out of the ycbY gene leads to loss of modification at G2445 and growth retardation. Growth competition with the parental wild-type strain leads to a gradual decrease in the knock-out strain cells proportion in the media	Escherichia coli
physiological function	it is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)