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Literature summary for 2.1.1.172 extracted from

  • Tscherne, J.S.; Nurse, K.; Popienick, P.; Ofengand, J.
    Purification, cloning, and characterization of the 16S RNA m2G1207 methyltransferase from Escherichia coli (1999), J. Biol. Chem., 274, 924-929.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, markedly reduces the level of methylation Escherichia coli
Mg2+ 6 mM, markedly reduces the level of methylation Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ close to unit stoichiometry of methylation can be achieved at 0.9 mM Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
gel filtration Escherichia coli
37600
-
1 * 37600, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine1207 in 16S rRNA Escherichia coli because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P39406
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine1207 in 16S rRNA because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
?
S-adenosyl-L-methionine + guanine1207 in 16S rRNA the enzyme reacts well with 30S subunits reconstituted from 16S RNA transcripts and 30S proteins but is almost inactive with the corresponding free RNA. Of the three naturally occurring N2-methylguanine residues, only N2-methylguanine1207 is formed. It is suggested that the optimal substrate may be a ribonucleoprotein particle less structured than a 30S ribosome but more so than free RNA. Localization of the site of methylation by hybridization-protection studies using deoxyoligomers that are complementary to the RNA sequence spanning each of the N2-methylguanine sites Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37600, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
16 S RNA m2G1207 methyltransferase
-
Escherichia coli
ribosomal RNA small subunit methyltransferase C
-
Escherichia coli
RsmC
-
Escherichia coli
yjjT
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli