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Literature summary for 2.1.1.171 extracted from
- Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure (2007), J. Biol. Chem., 282, 5880-5887.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| vapor diffusion method in hanging drops, structure determined and refined to 2.05 A | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine966 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0ADX9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + guanine966 in 16S rRNA = S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | proposed model of rRNA/RsmD interactions in the active site | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine966 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + guanine966 in 16S rRNA | RsmD protein efficiently methylates guanine966 of the assembled 30S subunits (purified from yhhF knock-out strain in vitro) in vitro in the presence of AdoMet. Protein-free 16 S rRNA was not a substrate for RsmD. The methylation is specific for guanine966 of 16S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine966 in 16S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| m2G966 methyltransferase | - |
Escherichia coli |
| RsmD | - |
Escherichia coli |
| yhhF | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | disruption of the yhhF gene by kanamycin resistance marker leads to a loss of modification at guanine966. Doubling of strain JW3430 carrying the inactive rsmD gene is essentially the same as that of the parental Escherichia coli strain BW25141 | Escherichia coli |
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Release 2023.1 (January 2023)
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