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Literature summary for 2.1.1.170 extracted from
- RsmG forms stable complexes with premature small subunit rRNA during bacterial ribosome biogenesis (2020), RSC Adv., 10, 22361-22369.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rsmG, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C172S/A14C | site-directed mutagenesis | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed | ? | - |
- |
 |
| additional information | Escherichia coli K12 | the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed | ? | - |
- |
|
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | Escherichia coli K12 | - |
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A6U5 | - |
- |
| Escherichia coli K12 | P0A6U5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed | Escherichia coli | ? | - |
- |
|
| additional information | RsmG enzyme binds to Escherichia coli small subunit precursor rRNAs. Ability of RsmG to bind various premature small subunit ribosomal RNAs with contrasting affinities, overview. Protein RsmG binds with approximately 15times higher affinity to premature 16S rRNA with the full leader sequence compared to that of mature 16S rRNA. Various r-proteins which bind to the 5'-domain influence RsmG binding. The binding cooperativity between RsmG and r-proteins is sensitive to the maturation status of premature small subunit rRNA. But neither the maturation of 16S rRNA nor the presence of various r-proteins significantly influence the methylation activity of RsmG | Escherichia coli | ? | - |
- |
|
| additional information | the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed | Escherichia coli K12 | ? | - |
- |
|
| additional information | RsmG enzyme binds to Escherichia coli small subunit precursor rRNAs. Ability of RsmG to bind various premature small subunit ribosomal RNAs with contrasting affinities, overview. Protein RsmG binds with approximately 15times higher affinity to premature 16S rRNA with the full leader sequence compared to that of mature 16S rRNA. Various r-proteins which bind to the 5'-domain influence RsmG binding. The binding cooperativity between RsmG and r-proteins is sensitive to the maturation status of premature small subunit rRNA. But neither the maturation of 16S rRNA nor the presence of various r-proteins significantly influence the methylation activity of RsmG | Escherichia coli K12 | ? | - |
- |
|
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | RsmG binds to 30S 5'-domain and methylates N7 of G527 that is located at the highly conserved 530 loop of 16S helix 18. Substrates rRNAs and are 5'-leader rRNA fragments | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | - |
Escherichia coli K12 | S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + guanine527 in 16S rRNA | RsmG binds to 30S 5'-domain and methylates N7 of G527 that is located at the highly conserved 530 loop of 16S helix 18. Substrates rRNAs and are 5'-leader rRNA fragments | Escherichia coli K12 | S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribosomal small subunit methyltransferase G | - |
Escherichia coli |
| rsmG | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is part of a SAM-dependent methyltransferase superfamily | Escherichia coli |
| additional information | ribosomal assembly proteins differentially stabilize RsmG complexes formed by mature and precursor 30S 5'-domain rRNA, positive and negative cooperativity, respectively, independency of the Mg2+ concentration, overview RsmG preferably binds to a native-like helix 18 structure | Escherichia coli |
| physiological function | protein RsmG is a methyltransferase enzyme that is responsible for N7 methylation in G527 of 16S rRNA. The capability of RsmG to bind to premature small subunit rRNA and alter its binding preference to various RNA-protein complexes based on the maturation of rRNA indicates its ability to influence ribosome assembly | Escherichia coli |
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