BRENDA - Enzyme Database show
show all sequences of 2.1.1.170

Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria

Okamoto, S.; Tamaru, A.; Nakajima, C.; Nishimura, K.; Tanaka, Y.; Tokuyama, S.; Suzuki, Y.; Ochi, K.; Mol. Microbiol. 63, 1096-1106 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
additional information
mutations within the gene gidB confer low-level streptomycin resistance. gidB mutations emerge spontaneously at a high frequency of 0.000001 and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli BW25113
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine527 in 16S rRNA
GidB is a m7G methyltransferase specific for 16S rRNA, identification of methylated nucleotide
705767
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine527 in 16S rRNA
GidB is a m7G methyltransferase specific for 16S rRNA, identification of methylated nucleotide
705767
Escherichia coli BW25113
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
mutations within the gene gidB confer low-level streptomycin resistance. gidB mutations emerge spontaneously at a high frequency of 0.000001 and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine527 in 16S rRNA
GidB is a m7G methyltransferase specific for 16S rRNA, identification of methylated nucleotide
705767
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine527 in 16S rRNA
GidB is a m7G methyltransferase specific for 16S rRNA, identification of methylated nucleotide
705767
Escherichia coli BW25113
S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
mutations within the gene gidB confer low-level streptomycin resistance. gidB Mmutations emerge spontaneously at a high frequency of 0.000001 and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutations within the gene gidB confer low-level streptomycin resistance. gidB Mmutations emerge spontaneously at a high frequency of 0.000001 and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains
Escherichia coli
Other publictions for EC 2.1.1.170
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733472
Benitez-Paez
Impairing methylations at ribo ...
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41-49
2014
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2
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1
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1
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1
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1
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2
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1
-
-
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-
-
-
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-
1
-
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-
-
-
-
-
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1
1
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721042
Benitez-Paez
Regulation of expression and c ...
Escherichia coli
RNA
18
795-806
2012
1
-
1
-
15
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
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1
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-
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1
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1
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1
1
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15
-
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1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
2
3
3
2
-
-
704222
Tanaka
Activation of secondary metabo ...
Streptomyces griseus, Streptomyces griseus IFO13189
J. Antibiot.
62
669-673
2009
-
-
-
-
-
-
-
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2
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2
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706725
Gregory
Structural and functional stud ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
RNA
15
1693-1704
2009
-
-
1
1
-
-
-
-
-
1
-
2
-
5
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704272
Nishimura
Mutations in rsmG, encoding a ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
J. Bacteriol.
189
3876-3883
2007
-
-
-
-
-
-
-
-
-
-
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6
-
-
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-
-
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-
-
-
-
-
-
-
1
1
-
-
-
704273
Nishimura
Identification of the RsmG met ...
Bacillus subtilis 168, Bacillus subtilis
J. Bacteriol.
189
6068-6073
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
90
-
-
-
-
-
-
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-
4
-
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2
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4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705767
Okamoto
Loss of a conserved 7-methylgu ...
Escherichia coli, Escherichia coli BW25113
Mol. Microbiol.
63
1096-1106
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
28
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
706646
Romanowski
Crystal structure of the Esche ...
Escherichia coli
Proteins
47
563-567
2002
-
-
1
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
1
-
1
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2
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-
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-
1
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-
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