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Literature summary for 2.1.1.168 extracted from

  • Pintard, L.; Bujnicki, J.M.; Lapeyre, B.; Bonnerot, C.
    MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase (2002), EMBO J., 21, 1139-1147.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.168

Cloned(Commentary)

Cloned (Comment) Organism
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 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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 Saccharomyces cerevisiae 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine2791 in 21S rRNA Saccharomyces cerevisiae mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA S-adenosyl-L-homocysteine + 2'-O-methyluridine2791 in 21S rRNA
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53123
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine2791 in 21S rRNA mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyluridine2791 in 21S rRNA
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 ?
S-adenosyl-L-methionine + uridine2791 in 21S rRNA Mrm2p methylates the 21S rRNA at position U2791 in vitro, when it is assembled with proteins into the ribosomal large subunit ir the precursor of the ribosomal large subunit. NO methylation is observed with deproteinized rRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyluridine2791 in 21S rRNA
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 ?

Synonyms

Synonyms Comment Organism
mitochondrial 21S rRNA methyltransferase
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 Saccharomyces cerevisiae
mitochondrial rRNA MTase 2
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 Saccharomyces cerevisiae
MRM2
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 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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 Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae Mrm2p is induced in glycerol-containing medium up

General Information

General Information Comment Organism
physiological function mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA Saccharomyces cerevisiae