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Literature summary for 2.1.1.166 extracted from

  • Caldas, T.; Binet, E.; Bouloc, P.; Richarme, G.
    Translational defects of Escherichia coli mutants deficient in the Um(2552) 23S ribosomal RNA methyltransferase RrmJ/FTSJ (2000), Biochem. Biophys. Res. Commun., 271, 714-718.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information rrmJ-deficient strain exhibit growth and translational defects compared to the wild-type strain. Growth rates of the rrmJ mutant are decreased at both low and high temperatures. Protein synthesis activity is reduced up to 65% when S30 rrmJ mutant extracts are tested in a coupled in vitro transcription/translation assay. In vitro methylation of these extracts by RrmJ partially restores protein synthesis activity Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140N5X9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine2552 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA
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 ?

Synonyms

Synonyms Comment Organism
FTSJ
-
 Escherichia coli
RrmJ
-
 Escherichia coli
Um(2552) 23S ribosomal RNA methyltransferase
-
 Escherichia coli
Um2552 methyltransferase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction rrmJ-deficient strain exhibit growth and translational defects compared to the wild-type strain. Growth rates of the rrmJ mutant are decreased at both low and high temperatures. Protein synthesis activity is reduced up to 65% when S30 rrmJ mutant extracts are tested in a coupled in vitro transcription/translation assay. In vitro methylation of these extracts by RrmJ partially restores protein synthesis activity Escherichia coli
physiological function the RrmJ-catalyzed methylation of Um2552 in 23S RNA strengthens ribosomal subunit interactions, increases protein synthesis activity, and improves cell growth rates even at non-heat shock temperatures Escherichia coli