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Literature summary for 2.1.1.165 extracted from

  • Nagatoshi. Y.; Nakamura, T.
    Characterization of three halide methyltransferases in Arabidopsis thaliana (2007), Plant Biotechnol., 24, 503-506.
No PubMed abstract available
Search for more literature for 2.1.1.165

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53300
-
 x * 53300, SDS-PAGE Arabidopsis thaliana
53500
-
 x * 53500, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O80562
-
-
Arabidopsis thaliana Q0WP12
-
-
Arabidopsis thaliana Q6AWU6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000058
-
 production of methyl iodide Arabidopsis thaliana
0.00009
-
 production of methyl iodide Arabidopsis thaliana
205
-
 production of methyl iodide Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + chloride
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl chloride
-
 ?
S-adenosyl-L-methionine + iodide recombinant protein methylates iodide with greater efficiency than chloride Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl iodide
-
 ?
S-adenosyl-L-methionine + iodide recombinant proteins methylate iodide with greater efficiency than chloride Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Subunits

Subunits Comment Organism
? x * 53500, SDS-PAGE Arabidopsis thaliana
? x * 53300, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtHOL1
-
 Arabidopsis thaliana
AtHOL2
-
 Arabidopsis thaliana
AtHOL3
-
 Arabidopsis thaliana