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Literature summary for 2.1.1.165 extracted from

  • Ni, X.; Hager, L.P
    Expression of Batis maritima methyl chloride transferase in Escherichia coli (1999), Proc. Natl. Acad. Sci. USA, 96, 3611-3615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.165

Activating Compound

Activating Compound Comment Organism Structure
Urea activity in 1, 2, and 3 M urea is about 1.75-, 2-, and 1.5-fold higher than in the absence of urea, respectively Batis maritima

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Batis maritima

Inhibitors

Inhibitors Comment Organism Structure
chloride competitive inhibition of methyl iodide formation Batis maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0294
-
 S-adenosyl-L-methionine pH 6.8, 22°C, native enzyme Batis maritima
0.23
-
 S-adenosyl-L-methionine pH 6.8, 22°C, recombinant enzyme Batis maritima
6.5
-
 Iodide pH 6.8, 22°C, recombinant enzyme Batis maritima
8.5
-
 Iodide pH 6.8, 22°C, native enzyme Batis maritima
18.5
-
 bromide pH 6.8, 22°C, native enzyme Batis maritima
25
-
 bromide pH 6.8, 22°C, recombinant enzyme Batis maritima
100
-
 chloride pH 6.8, 22°C, recombinant enzyme Batis maritima
155
-
 chloride pH 6.8, 22°C, native enzyme Batis maritima

Metals/Ions

Metals/Ions Comment Organism Structure
ammonium sulfate the activity of the recombinant methylase in 0.5 M and 1.0 M ammonium sulfate is about 1.25fold higher than in the absence of ammonium sulfate Batis maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + chloride Batis maritima this enzyme possibly functions in the control and regulation of the internal concentration of chloride ions in halophytic plant cells S-adenosyl-L-homocysteine + methyl chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Batis maritima Q9ZSZ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + bromide
-
 Batis maritima S-adenosyl-L-homocysteine + methyl bromide
-
 ?
S-adenosyl-L-methionine + chloride
-
 Batis maritima S-adenosyl-L-homocysteine + methyl chloride
-
 ?
S-adenosyl-L-methionine + chloride this enzyme possibly functions in the control and regulation of the internal concentration of chloride ions in halophytic plant cells Batis maritima S-adenosyl-L-homocysteine + methyl chloride
-
 ?
S-adenosyl-L-methionine + iodide
-
 Batis maritima S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Synonyms

Synonyms Comment Organism
methyl chloride transferase
-
 Batis maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 methyl bromide production Batis maritima
6.2 6.8 methyl chloride production Batis maritima
6.8 7.5 methyl iodide production Batis maritima