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Literature summary for 2.1.1.163 extracted from

  • Xie, F.; Cheng, G.; Xu, H.; Wang, Z.; Lei, L.; Li, Y.
    Identification of a novel gene for biosynthesis of a bacteroid-specific electron carrier menaquinone (2011), PLoS ONE, 6, e28995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dmtH, cloned from a transposon-inserted mutant library, DNA and mino acid sequence determination and analysis. Real-time quantitative PCR shows that dmtH is a bacteroid-specific gene with highest expression at 25 days after inoculation of strain 7653R Mesorhizobium huakuii

Protein Variants

Protein Variants Comment Organism
additional information construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R Mesorhizobium huakuii

Organism

Organism UniProt Comment Textmining
Mesorhizobium huakuii
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gene dmtH, isolated from a transposon-inserted mutant library
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Mesorhizobium huakuii 7653R
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gene dmtH, isolated from a transposon-inserted mutant library
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no activity in Rhizobium sp.
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-
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Synonyms

Synonyms Comment Organism
DMK methyltransferase
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Mesorhizobium huakuii
DmtH protein
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Mesorhizobium huakuii

General Information

General Information Comment Organism
metabolism demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R Mesorhizobium huakuii
physiological function the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation Mesorhizobium huakuii