Cloned (Comment) | Organism |
---|---|
gene dmtH, cloned from a transposon-inserted mutant library, DNA and mino acid sequence determination and analysis. Real-time quantitative PCR shows that dmtH is a bacteroid-specific gene with highest expression at 25 days after inoculation of strain 7653R | Mesorhizobium huakuii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R | Mesorhizobium huakuii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium huakuii | - |
gene dmtH, isolated from a transposon-inserted mutant library | - |
Mesorhizobium huakuii 7653R | - |
gene dmtH, isolated from a transposon-inserted mutant library | - |
no activity in Rhizobium sp. | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
DMK methyltransferase | - |
Mesorhizobium huakuii |
DmtH protein | - |
Mesorhizobium huakuii |
General Information | Comment | Organism |
---|---|---|
metabolism | demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R | Mesorhizobium huakuii |
physiological function | the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation | Mesorhizobium huakuii |