Any feedback?
Literature summary for 2.1.1.160 extracted from
- Separation of the N-7 methyltransferase, the key enzyme in caffeine biosynthesis (1997), Phytochemistry, 45, 1407-1414.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Coffea arabica |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Coffea arabica |
| 67000 | - |
about, gel filtration | Coffea arabica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea arabica | last step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coffea arabica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 24fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration | Coffea arabica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | young | Coffea arabica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.65 | - |
purified enzyme | Coffea arabica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
| S-adenosyl-L-methionine + 3,7-dimethylxanthine | last step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Coffea arabica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1NMT | - |
Coffea arabica |
| N-1 methyltransferase | - |
Coffea arabica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Coffea arabica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Coffea arabica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Coffea arabica | chromatofocusing | 4.9 | 4 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
