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Literature summary for 2.1.1.160 extracted from

  • Roberts, M.F.; Waller, G.R.
    N-methyltransferases and 7-methyl-N9-nucleoside hydrolase activity in Coffea arabica and the biosynthesis of caffeine (1979), Phytochemistry, 18, 451-455.
No PubMed abstract available
Search for more literature for 2.1.1.160

Activating Compound

Activating Compound Comment Organism Structure
paraxanthine i.e. 1,7-dimethylxanthine, slight stimulation Coffea arabica

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 0.5 mM, 23% inhibition at 0.05 mM Coffea arabica
azouracil 18% inhibition at 0.1 mM, no inhibition at 1 mM Coffea arabica
iodoacetate complete inhibition at 100 mM, 89% inhibition at 10 mM Coffea arabica
KCN 74% inhibition at 100 mM, but 33% activation at 10 mM Coffea arabica
additional information no inhibition by EDTA at 0.5-5 mM Coffea arabica
N-Methylmaleimide complete inhibition at 1 mM, no inhibition at 0.1 mM Coffea arabica
NaN3 22% inhibition at 10 mM, 16% at 1 mM Coffea arabica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 S-adenosyl-L-methionine pH 8.5, 30°C Coffea arabica
0.2
-
 Theobromine pH 8.5, 30°C Coffea arabica

Metals/Ions

Metals/Ions Comment Organism Structure
KCN 74% inhibition at 100 mM, but 33% activation at 10 mM Coffea arabica
Mg2+ activates Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coffea arabica feeding experiments with fruit samples, determination of reaction products, overview ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica last step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

Organism UniProt Comment Textmining
Coffea arabica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit unripe, green Coffea arabica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, overview Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information feeding experiments with fruit samples, determination of reaction products, overview Coffea arabica ?
-
 ?
additional information substrate specificity, overview, no activity with xanthine, hypoxanthine, and xanthosine Coffea arabica ?
-
 ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine last step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Coffea arabica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 narrow pH optimum Coffea arabica

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Coffea arabica