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Literature summary for 2.1.1.159 extracted from

  • Schimpl, F.C.; Kiyota, E.; Mayer, J.L.; Goncalves, J.F.; da Silva, J.F.; Mazzafera, P.
    Molecular and biochemical characterization of caffeine synthase and purine alkaloid concentration in guarana fruit (2014), Phytochemistry, 105, 25-36.
    View publication on PubMed
Search for more literature for 2.1.1.159

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-pRil cells Paullinia cupana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.258
-
 3,7-Dimethylxanthine native enzyme from seeds, at pH 8.0 and 28°C Paullinia cupana
0.277
-
 3,7-Dimethylxanthine recombinant enzyme, at pH 8.0 and 28°C Paullinia cupana
0.328
-
 7-methylxanthine recombinant enzyme, at pH 8.0 and 28°C Paullinia cupana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40100
-
 x * 40100, calculated from amino acid sequence Paullinia cupana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine Paullinia cupana
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 7-methylxanthine Paullinia cupana best substrate S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Organism

Organism UniProt Comment Textmining
Paullinia cupana
-
 variant Paullinia cupana sorbilis, cultivars BRS-Amazonas, BRS-Maues, BRS-Luzeia, BRS-CG372, and BRS-CG611
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by Ni-NTA agarose column chromatography Paullinia cupana

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Paullinia cupana
-
inflorescence
-
 Paullinia cupana
-
leaf
-
 Paullinia cupana
-
pericarp
-
 Paullinia cupana
-
seed highest expression Paullinia cupana
-
stem
-
 Paullinia cupana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Paullinia cupana S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 7-methylxanthine best substrate Paullinia cupana S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Subunits

Subunits Comment Organism
? x * 40100, calculated from amino acid sequence Paullinia cupana

pI Value

Organism Comment pI Value Maximum pI Value
Paullinia cupana calculated from amino acid sequence
-
 5.1