Cloned (Comment) | Organism |
---|---|
isozyme MXMT1, DNA and amnio acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
isozyme MXMT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3) | Coffea arabica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
S-adenosyl-L-methionine | recombinant MXMT1 | Coffea arabica | |
0.014 | - |
S-adenosyl-L-methionine | recombinant MXMT2 | Coffea arabica | |
0.148 | - |
7-methylxanthine | recombinant MXMT1 | Coffea arabica | |
0.251 | - |
7-methylxanthine | recombinant MXMT2 | Coffea arabica | |
0.458 | - |
paraxanthine | recombinant MXMT1 | Coffea arabica | |
0.738 | - |
paraxanthine | recombinant MXMT2 | Coffea arabica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43400 | - |
2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
74000 | - |
recombinant isozyme MXMT2, gel filtration | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + paraxanthine | Coffea arabica | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | Q84PP7 | isozyme MXMT2; isozyme MXMT2 | - |
Coffea arabica | Q9AVJ9 | isozyme MXMT1; isozyme MXTM1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | isozyme MXMT2 | Coffea arabica | - |
flower bud | isozyme MXTM1 | Coffea arabica | - |
fruit | immature, isozyme MXMT2 | Coffea arabica | - |
fruit | immature, isozyme MXTM1 | Coffea arabica | - |
leaf | high expression in young leaves of isozyme MXTM2, low expression level in expanded leaves | Coffea arabica | - |
leaf | very high expression in young leaves of isozyme MXTM1, low expression level in expanded leaves | Coffea arabica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 20fold higher activity by isozyme MXMT2 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | preferred substrate, 3fold higher activity by isozyme MXMT1 compared to activity with paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + paraxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT1 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
S-adenosyl-L-methionine + paraxanthine | low activity by isozyme MXMT2 | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43400, recombinant isozyme MXMT2, SDS-PAGE | Coffea arabica |
Synonyms | Comment | Organism |
---|---|---|
MXMT1 | - |
Coffea arabica |
MXMT2 | - |
Coffea arabica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Coffea arabica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Coffea arabica |