Activating Compound | Comment | Organism | Structure |
---|---|---|---|
paraxanthine | i.e. 1,7-dimethylxanthine, slight stimulation | Coffea arabica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 0.5 mM, 30% inhibition at 0.05 mM | Coffea arabica | |
azouracil | 20% inhibition at 0.1 mM, 28% inhibition at 1 mM | Coffea arabica | |
iodoacetate | complete inhibition at 100 mM, 79% inhibition at 10 mM | Coffea arabica | |
KCN | 88% inhibition at 100 mM, 44% inhibition at 10 mM | Coffea arabica | |
additional information | no or poor inhibition by EDTA at 0.5-5 mM | Coffea arabica | |
N-Methylmaleimide | complete inhibition at 1 mM, 17% inhibition at 0.1 mM | Coffea arabica | |
NaN3 | 39% inhibition at 10 mM, 10% at 1 mM | Coffea arabica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-methionine | pH 8.5, 30°C | Coffea arabica | |
0.07 | - |
paraxanthine | pH 8.5, 30°C | Coffea arabica | |
0.2 | - |
7-methylxanthine | pH 8.5, 30°C | Coffea arabica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Coffea arabica | feeding experiments with fruit samples, determination of reaction products, overview | ? | - |
? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | Coffea arabica | i.e. paraxanthine | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | unripe, green | Coffea arabica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, overview | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | feeding experiments with fruit samples, determination of reaction products, overview | Coffea arabica | ? | - |
? | |
additional information | substrate specificity, overview, no activity with xanthine, hypoxanthine, and xanthosine | Coffea arabica | ? | - |
? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coffea arabica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
narrow pH optimum | Coffea arabica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Coffea arabica |