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Literature summary for 2.1.1.159 extracted from

  • Kato, M.; Mizuno, K.; Crozier, A.; Fujimura, T.; Ashihara, H.
    Caffeine synthase gene from tea leaves (2000), Nature, 406, 956-957.
    View publication on PubMed

Application

Application Comment Organism
biotechnology large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea Camellia sinensis
food industry large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea Camellia sinensis

Cloned(Commentary)

Cloned (Comment) Organism
gene TCS1, cDNA and amino acid sequence determination and analysis, expression in Escherichia coli Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Camellia sinensis the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 ?
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis reaction of EC 2.1.1.160 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q9FZN8 gene TCS1; bifunctional enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Camellia sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
recombinant enzyme in Escherichia coli cell extract, substrate 7-methylxanthine Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 Camellia sinensis ?
-
?
additional information substrate specificity, overview, the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160, no activity with xanthosine and theophylline Camellia sinensis ?
-
?
S-adenosyl-L-methionine + 1-methylxanthine low activity with the recombinant enzyme, poor activity with the native enzyme Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
S-adenosyl-L-methionine + 3,7-dimethylxanthine reaction of EC 2.1.1.160 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 3-methylxanthine poor activity with the recombinant enzyme, low activity with the native enzyme Camellia sinensis S-adenosyl-L-homocysteine + 1,3-dimethylxanthine i.e. theophylline ?
S-adenosyl-L-methionine + 7-methylxanthine
-
Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
S-adenosyl-L-methionine + paraxanthine best substrate Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Synonyms

Synonyms Comment Organism
caffeine synthase
-
Camellia sinensis
CS
-
Camellia sinensis
TCS1
-
Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Camellia sinensis