Application | Comment | Organism |
---|---|---|
biotechnology | large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea | Camellia sinensis |
food industry | large-scale production of transgenic enzyme-deficient Coffea arabica and Camellia sinensis plants are a practical possibilty for production of decaffeinated coffee or tea | Camellia sinensis |
Cloned (Comment) | Organism |
---|---|
gene TCS1, cDNA and amino acid sequence determination and analysis, expression in Escherichia coli | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Camellia sinensis | the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 | ? | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | Camellia sinensis | reaction of EC 2.1.1.160 | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | Q9FZN8 | gene TCS1; bifunctional enzyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
recombinant enzyme in Escherichia coli cell extract, substrate 7-methylxanthine | Camellia sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 | Camellia sinensis | ? | - |
? | |
additional information | substrate specificity, overview, the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160, no activity with xanthosine and theophylline | Camellia sinensis | ? | - |
? | |
S-adenosyl-L-methionine + 1-methylxanthine | low activity with the recombinant enzyme, poor activity with the native enzyme | Camellia sinensis | S-adenosyl-L-homocysteine + 1,3-dimethylxanthine | i.e. theophylline | ? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | reaction of EC 2.1.1.160 | Camellia sinensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 3-methylxanthine | poor activity with the recombinant enzyme, low activity with the native enzyme | Camellia sinensis | S-adenosyl-L-homocysteine + 1,3-dimethylxanthine | i.e. theophylline | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? | |
S-adenosyl-L-methionine + paraxanthine | best substrate | Camellia sinensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? |
Synonyms | Comment | Organism |
---|---|---|
caffeine synthase | - |
Camellia sinensis |
CS | - |
Camellia sinensis |
TCS1 | - |
Camellia sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Camellia sinensis |