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show all sequences of 2.1.1.158

Cloning, expression, crystallization and preliminary x-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta)

McCarthy, A.A.; Biget, L.; Lin, C.; Petiard, V.; Tanksley, S.D.; McCarthy, J.G.; Acta Crystallogr. Sect. F F63, 304-307 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of His-tagged XMT1 in Escherichia coli strain BL21(DE3)
Coffea canephora
Crystallization (Commentary)
Crystallization
Organism
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution
Coffea canephora
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, about, sequence calculation
Coffea canephora
80000
-
about, recombinant enzyme, gel filtration
Coffea canephora
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea canephora
-
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coffea canephora
A4GE69
XMT1; var. Robusta
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration
Coffea canephora
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
667101
Coffea canephora
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 42000, about, sequence calculation
Coffea canephora
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of His-tagged XMT1 in Escherichia coli strain BL21(DE3)
Coffea canephora
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution
Coffea canephora
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, about, sequence calculation
Coffea canephora
80000
-
about, recombinant enzyme, gel filtration
Coffea canephora
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea canephora
-
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration
Coffea canephora
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
667101
Coffea canephora
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 42000, about, sequence calculation
Coffea canephora
Other publictions for EC 2.1.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721124
Mizuno
Essential region for 3-N methy ...
Coffea sp.
Z. Naturforsch. C
65c
257-265
2010
-
-
1
-
16
-
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1
-
1
-
2
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2
-
1
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-
1
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-
1
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-
1
1
-
16
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
696486
Kodama
Dimerization of N-methyltransf ...
Coffea arabica
Biochimie
90
547-551
2008
-
-
-
-
-
-
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-
1
-
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1
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1
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700656
Deng
-
Fine control of caffeine biosy ...
Camellia sinensis
Phytochem. Lett.
1
195-198
2008
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1
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-
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-
1
1
-
-
-
667101
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
F63
304-307
2007
-
-
1
1
-
-
-
-
-
-
2
1
-
1
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-
1
-
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1
1
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1
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1
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2
1
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1
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1
1
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-
-
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-
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670598
McCarthy
The structure of two N-methylt ...
Coffea canephora
Plant Physiol.
144
879-889
2007
-
-
1
1
-
-
-
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1
-
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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684218
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
63
304-307
2007
-
-
1
1
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2
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1
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1
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1
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1
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1
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1
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1
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670621
Koshiro
-
Changes in content and biosynt ...
Coffea arabica, Coffea canephora
Plant Sci.
171
242-250
2006
-
-
-
-
-
-
-
-
-
-
-
6
-
2
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-
8
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9
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6
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8
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9
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670568
Uefuji
Caffeine production in tobacco ...
Coffea arabica
Plant Mol. Biol.
59
221-227
2005
-
1
1
-
1
-
-
-
-
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1
-
3
-
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1
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1
1
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1
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1
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1
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670565
Ogita
Application of RNAi to confirm ...
Coffea arabica, Coffea canephora
Plant Mol. Biol.
54
931-941
2004
-
-
2
-
2
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2
-
6
-
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6
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4
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2
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2
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2
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6
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4
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668586
Mizuno
The first committed step react ...
Coffea arabica
FEBS Lett.
547
56-60
2003
-
-
1
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1
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1
-
4
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4
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3
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1
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1
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1
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4
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3
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670578
Uefuji
Molecular cloning and function ...
Coffea arabica
Plant Physiol.
132
372-380
2003
-
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1
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1
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2
1
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1
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3
1
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1
1
1
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1
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1
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1
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1
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3
1
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1
1
1
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1
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655545
Koshiishi
A new caffeine biosynthetic pa ...
Camellia sinensis
FEBS Lett.
499
50-54
2001
-
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1
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2
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1
1
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1
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1
1
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2
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1
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-
1
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669194
Ogawa
7-Methylxanthine methyltransfe ...
Coffea arabica
J. Biol. Chem.
276
8213-8218
2001
-
-
1
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-
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1
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1
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3
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2
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1
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1
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3
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2
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667105
Moisyadi
-
Cloning and characterization o ...
Coffea arabica
Acta Hortic.
461
367-377
1998
-
-
1
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1
1
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1
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1
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2
1
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1
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1
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1
1
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1
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2
1
-
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-
1
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-
670509
Waldhauser
Separation of the N-7 methyltr ...
Coffea arabica
Phytochemistry
45
1407-1414
1997
-
-
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-
-
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1
2
1
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2
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1
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1
1
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2
1
1
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1
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1
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1
2
1
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1
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1
1
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2
1
1
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1
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1
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-
667116
Negishi
-
The role of xanthosine in the ...
Coffea arabica
Agric. Biol. Chem.
49
2221-2222
1985
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1
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1
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1
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5
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2
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1
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1
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1
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1
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5
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1
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1
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