BRENDA - Enzyme Database
show all sequences of 2.1.1.158

Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants

Uefuji, H.; Ogita, S.; Yamaguchi, Y.; Koizumi, N.; Sano, H.; Plant Physiol. 132, 372-380 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
isozyme XMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3)
Coffea arabica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.078
-
Xanthosine
recombinant XMT1
Coffea arabica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41800
-
2 * 41800, recombinant isozyme XMT1, SDS-PAGE
Coffea arabica
69000
-
recombinant isozyme XMT1, gel filtration
Coffea arabica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea arabica
-
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coffea arabica
Q9AVK0
isozyme XMT1; isozyme XMT1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower bud
high expression
Coffea arabica
-
fruit
high expression level in immature fruits, low expression in mature fruits
Coffea arabica
-
leaf
very high expression in young leaves, moderate expression in expanded leaves
Coffea arabica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
recombinant enzyme
Coffea arabica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
670578
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 41800, recombinant isozyme XMT1, SDS-PAGE
Coffea arabica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Coffea arabica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Coffea arabica
Cloned(Commentary) (protein specific)
Commentary
Organism
isozyme XMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3)
Coffea arabica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.078
-
Xanthosine
recombinant XMT1
Coffea arabica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41800
-
2 * 41800, recombinant isozyme XMT1, SDS-PAGE
Coffea arabica
69000
-
recombinant isozyme XMT1, gel filtration
Coffea arabica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea arabica
-
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower bud
high expression
Coffea arabica
-
fruit
high expression level in immature fruits, low expression in mature fruits
Coffea arabica
-
leaf
very high expression in young leaves, moderate expression in expanded leaves
Coffea arabica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
recombinant enzyme
Coffea arabica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
670578
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 41800, recombinant isozyme XMT1, SDS-PAGE
Coffea arabica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Coffea arabica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Coffea arabica
Other publictions for EC 2.1.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721124
Mizuno
Essential region for 3-N methy ...
Coffea sp.
Z. Naturforsch. C
65c
257-265
2010
-
-
1
-
16
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
16
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
696486
Kodama
Dimerization of N-methyltransf ...
Coffea arabica
Biochimie
90
547-551
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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-
-
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-
-
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-
-
1
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700656
Deng
-
Fine control of caffeine biosy ...
Camellia sinensis
Phytochem. Lett.
1
195-198
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
667101
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
F63
304-307
2007
-
-
1
1
-
-
-
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670598
McCarthy
The structure of two N-methylt ...
Coffea canephora
Plant Physiol.
144
879-889
2007
-
-
1
1
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
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-
-
1
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-
-
-
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-
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-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
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-
-
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-
-
-
1
-
1
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-
-
1
-
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-
-
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-
-
-
-
684218
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
63
304-307
2007
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670621
Koshiro
-
Changes in content and biosynt ...
Coffea arabica, Coffea canephora
Plant Sci.
171
242-250
2006
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
8
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
6
-
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-
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8
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670568
Uefuji
Caffeine production in tobacco ...
Coffea arabica
Plant Mol. Biol.
59
221-227
2005
-
1
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
-
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-
-
1
1
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-
1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670565
Ogita
Application of RNAi to confirm ...
Coffea arabica, Coffea canephora
Plant Mol. Biol.
54
931-941
2004
-
-
2
-
2
-
-
-
-
-
-
2
-
6
-
-
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-
-
6
-
-
4
-
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-
-
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-
-
-
-
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-
2
-
-
2
-
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-
2
-
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-
6
-
-
4
-
-
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-
-
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-
-
-
-
-
-
-
-
668586
Mizuno
The first committed step react ...
Coffea arabica
FEBS Lett.
547
56-60
2003
-
-
1
-
-
-
-
1
-
-
-
1
-
4
-
-
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-
-
4
-
-
3
-
-
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-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
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1
-
-
-
1
-
-
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-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670578
Uefuji
Molecular cloning and function ...
Coffea arabica
Plant Physiol.
132
372-380
2003
-
-
1
-
-
-
-
1
-
-
2
1
-
1
-
-
-
-
-
3
1
-
1
1
1
-
-
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
-
2
1
-
-
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-
-
3
1
-
1
1
1
-
-
-
1
-
-
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-
-
-
-
-
-
655545
Koshiishi
A new caffeine biosynthetic pa ...
Camellia sinensis
FEBS Lett.
499
50-54
2001
-
-
-
-
-
-
-
-
-
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-
1
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2
-
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-
1
1
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2
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1
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-
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1
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-
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1
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-
1
1
-
2
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
669194
Ogawa
7-Methylxanthine methyltransfe ...
Coffea arabica
J. Biol. Chem.
276
8213-8218
2001
-
-
1
-
-
-
-
-
-
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1
-
1
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3
-
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2
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1
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1
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3
-
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2
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-
-
-
-
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-
-
-
-
-
-
667105
Moisyadi
-
Cloning and characterization o ...
Coffea arabica
Acta Hortic.
461
367-377
1998
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
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-
-
-
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-
-
1
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-
1
-
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-
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-
-
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-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
670509
Waldhauser
Separation of the N-7 methyltr ...
Coffea arabica
Phytochemistry
45
1407-1414
1997
-
-
-
-
-
-
-
-
-
1
2
1
-
2
-
-
1
-
-
1
1
-
2
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
-
1
2
1
-
-
-
1
-
1
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
667116
Negishi
-
The role of xanthosine in the ...
Coffea arabica
Agric. Biol. Chem.
49
2221-2222
1985
-
-
-
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1
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1
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1
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5
-
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2
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1
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1
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1
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1
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5
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2
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1
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1
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