Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.157 extracted from

  • Chen, S.; Lai, M.; Lai, S.; Lee, Y.
    Characterization of osmolyte betaine synthesizing sarcosine dimethylglycine N-methyltransferase from Methanohalophilus portucalensis (2009), Arch. Microbiol., 191, 735-743.
    View publication on PubMed
Search for more literature for 2.1.1.157

Inhibitors

Inhibitors Comment Organism Structure
betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis
0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis
2.29
-
 sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
3.76
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel fitlration Methanohalophilus portucalensis

Organism

Organism UniProt Comment Textmining
Methanohalophilus portucalensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanohalophilus portucalensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis
3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis

Storage Stability

Storage Stability Organism
-85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine + H+
-
 ?
S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
2.68
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis

pI Value

Organism Comment pI Value Maximum pI Value
Methanohalophilus portucalensis isoelectric focusing
-
 5