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Literature summary for 2.1.1.157 extracted from

  • McCoy, J.G.; Bailey, L.J.; Ng, Y.H.; Bingman, C.A.; Wrobel, R.; Weber, A.P.; Fox, B.G.; Phillips, G.N.
    Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria (2008), Proteins, 74, 368-367.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.157

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Galdieria sulphuraria

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor-diffusion method, crystal structure of the enzyme, solved to a resolution of 1.95 A Galdieria sulphuraria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.144
-
 S-adenosyl-L-methionine cosubstrate: sarcosine Galdieria sulphuraria
0.15
-
 S-adenosyl-L-methionine cosubstrate: N,N-dimethylglycine Galdieria sulphuraria
2
-
 sarcosine
-
 Galdieria sulphuraria
2.8
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, denaturing PAGE Galdieria sulphuraria
67700
-
 gel filtration Galdieria sulphuraria

Organism

Organism UniProt Comment Textmining
Galdieria sulphuraria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Galdieria sulphuraria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + sarcosine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, denaturing PAGE Galdieria sulphuraria

Synonyms

Synonyms Comment Organism
dimethylglycine methyltransferase
-
 Galdieria sulphuraria
GsSDMT
-
 Galdieria sulphuraria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61
-
 melting temperature Galdieria sulphuraria

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.07
-
 sarcosine
-
 Galdieria sulphuraria
1.4
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 methylation of dimethylglycine Galdieria sulphuraria
8
-
 methylation of sarcosine Galdieria sulphuraria