Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.157 extracted from

  • Nyyssola, A.; Kerovuo, J.; Kaukinen, P.; von Weymarn, N.; Reinikainen, T.
    Extreme halophiles synthesize betaine from glycine by methylation (2000), J. Biol. Chem., 275, 22196-22201.
    View publication on PubMed
Search for more literature for 2.1.1.157

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, the single gene encodes the sarcosine dimethylglycine methyltransferase as well as the glycine sarcosine methyltransferase, EC 2.1.1.156, the first being probably a proteolytic processing product of the latter, functional expression of a truncated soluble enzyme form in Escherichia coli Actinopolyspora halophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Actinopolyspora halophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Halorhodospira halochloris the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process ?
-
 ?
additional information Actinopolyspora halophila the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process ?
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine Halorhodospira halochloris
-
 S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine Actinopolyspora halophila
-
 S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + sarcosine Halorhodospira halochloris
-
 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
S-adenosyl-L-methionine + sarcosine Actinopolyspora halophila
-
 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Organism

Organism UniProt Comment Textmining
Actinopolyspora halophila
-
 ATCC 27976
-
Halorhodospira halochloris
-
-
-
Halorhodospira halochloris Q9KJ21 i.e. Halorhodospira halochloris
-

Purification (Commentary)

Purification (Comment) Organism
by AMP affinity chromatography and ultrafiltration Actinopolyspora halophila

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine (overall reaction) Halorhodospira halochloris
a
S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine + H+ (1b) Halorhodospira halochloris
a
S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine (1a) Halorhodospira halochloris
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
 activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts Halorhodospira halochloris
0.34
-
 activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts Actinopolyspora halophila
0.36
-
 activity with sarcosine and S-adenosyl-L-methionine, purified enzyme Actinopolyspora halophila
0.93
-
 activity with N,N-dimethylglycine and S-adenosyl-L-methionine in crude cell extracts Actinopolyspora halophila
1
-
 activity with N,N-dimethylglycine and S-adenosyl-L-methionine, purified enzyme Actinopolyspora halophila
2.6
-
 activity with N,N-dimethylglycine and S-adenosyl-L-methionine in crude cell extracts Halorhodospira halochloris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + sarcosine (overall reaction) Halorhodospira halochloris 2 S-adenosyl-L-homocysteine + betaine
-
 ?
additional information the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process Halorhodospira halochloris ?
-
 ?
additional information the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process Actinopolyspora halophila ?
-
 ?
additional information the enzyme has overlapping activities with the enzyme catalyzing the prior reaction steps Halorhodospira halochloris ?
-
 ?
additional information the enzyme has overlapping activities with the enzyme catalyzing the prior reaction steps Actinopolyspora halophila ?
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Halorhodospira halochloris S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Actinopolyspora halophila S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Halorhodospira halochloris S-adenosyl-L-homocysteine + betaine i.e. N,N,N-trimethylglycine ?
S-adenosyl-L-methionine + N,N-dimethylglycine preferred substrate Actinopolyspora halophila S-adenosyl-L-homocysteine + betaine i.e. N,N,N-trimethylglycine ?
S-adenosyl-L-methionine + N,N-dimethylglycine (1b) Halorhodospira halochloris S-adenosyl-L-homocysteine + betaine
-
 ?
S-adenosyl-L-methionine + sarcosine
-
 Halorhodospira halochloris S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
S-adenosyl-L-methionine + sarcosine
-
 Actinopolyspora halophila S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
S-adenosyl-L-methionine + sarcosine (1a) Halorhodospira halochloris S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Actinopolyspora halophila

Synonyms

Synonyms Comment Organism
sarcosine dimethylglycine methyltransferase
-
 Halorhodospira halochloris
sarcosine dimethylglycine methyltransferase
-
 Actinopolyspora halophila
SDMT
-
 Halorhodospira halochloris
SDMT
-
 Actinopolyspora halophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Halorhodospira halochloris
37
-
 assay at Actinopolyspora halophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Halorhodospira halochloris
7.5
-
 assay at Actinopolyspora halophila