Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, functional coexpression in Escherichia coli with sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, conferring betaine accumulation and increased salt tolerance to the Escherichia coli cells | Halorhodospira halochloris |
DNA and amino acid sequence determination, the single gene encodes the glycine sarcosine methyltransferase as well as the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, the latter being probably a proteolytic processing product of the first, poor expression in Escherichia coli of the full length and the truncated enzyme | Actinopolyspora halophila |
General Stability | Organism |
---|---|
enzyme is instable during purification, activity is lost within 30 min | Actinopolyspora halophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Halorhodospira halochloris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Halorhodospira halochloris | the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process | ? | - |
? | |
additional information | Actinopolyspora halophila | the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process | ? | - |
? | |
S-adenosyl-L-methionine + glycine | Halorhodospira halochloris | - |
S-adenosyl-L-homocysteine + sarcosine | - |
? | |
S-adenosyl-L-methionine + glycine | Actinopolyspora halophila | - |
S-adenosyl-L-homocysteine + sarcosine | - |
? | |
S-adenosyl-L-methionine + sarcosine | Halorhodospira halochloris | - |
S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
S-adenosyl-L-methionine + sarcosine | Actinopolyspora halophila | - |
S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinopolyspora halophila | Q9KJ20 | - |
- |
Halorhodospira halochloris | - |
- |
- |
Halorhodospira halochloris | Q9KJ22 | i.e. Halorhodospira halochloris, ATCC 35916 | - |
Purification (Comment) | Organism |
---|---|
by hydrophobic interaction chromatography, ammonium sulfate fractionation, gel filtration, ion exchange chromatography and ultrafiltration | Halorhodospira halochloris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
activity with glycine and S-adenosyl-L-methionine in crude cell extracts | Actinopolyspora halophila |
0.058 | - |
activity with glycine and S-adenosyl-L-methionine in crude cell extracts | Halorhodospira halochloris |
0.19 | - |
activity with sarcosine and S-adenosyl-L-methionine, purified enzyme | Halorhodospira halochloris |
0.23 | - |
activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts | Halorhodospira halochloris |
0.34 | - |
activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts | Actinopolyspora halophila |
0.52 | - |
activity with glycine and S-adenosyl-L-methionine, purified enzyme | Halorhodospira halochloris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + glycine | overall reaction | Halorhodospira halochloris | 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
additional information | the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process | Halorhodospira halochloris | ? | - |
? | |
additional information | the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process | Actinopolyspora halophila | ? | - |
? | |
additional information | the enzyme has overlapping activities with the enzyme catalyzing the following reaction steps | Halorhodospira halochloris | ? | - |
? | |
additional information | the enzyme has overlapping activities with the enzyme catalyzing the following reaction steps | Actinopolyspora halophila | ? | - |
? | |
S-adenosyl-L-methionine + glycine | - |
Halorhodospira halochloris | S-adenosyl-L-homocysteine + sarcosine | - |
? | |
S-adenosyl-L-methionine + glycine | - |
Actinopolyspora halophila | S-adenosyl-L-homocysteine + sarcosine | - |
? | |
S-adenosyl-L-methionine + glycine | - |
Halorhodospira halochloris | S-adenosyl-L-homocysteine + sarcosine | i.e. 2-methylglycine | ? | |
S-adenosyl-L-methionine + sarcosine | - |
Halorhodospira halochloris | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
S-adenosyl-L-methionine + sarcosine | - |
Actinopolyspora halophila | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
S-adenosyl-L-methionine + sarcosine | i.e. 2-methylglycine | Actinopolyspora halophila | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Halorhodospira halochloris |
Synonyms | Comment | Organism |
---|---|---|
glycine sarcosine methyltransferase | - |
Halorhodospira halochloris |
glycine sarcosine methyltransferase | - |
Actinopolyspora halophila |
GSMT | - |
Halorhodospira halochloris |
GSMT | - |
Actinopolyspora halophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Halorhodospira halochloris |
37 | - |
assay at | Actinopolyspora halophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Halorhodospira halochloris |
7.5 | - |
assay at | Actinopolyspora halophila |