Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | increases activity | Helicobacter pylori | |
FMN | increases activity | Helicobacter pylori | |
UMP | - |
Helicobacter pylori |
Application | Comment | Organism |
---|---|---|
medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Rickettsia prowazekii |
medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Borreliella burgdorferi |
medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Campylobacter jejuni |
medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
complemetation of Escherichia coli, transformants are able to grow in the absence of thymidine | Helicobacter pylori |
complemetation of Escherichia coli, transformants are unable to grow in the absence of thymidine | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
S107A | no activity | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Helicobacter pylori |
31500 | - |
calculated from DNA sequence | Helicobacter pylori |
111000 | - |
gel filtration | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Dictyostelium discoideum | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Mycobacterium tuberculosis | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Saccharolobus solfataricus | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Pyrococcus horikoshii | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Treponema pallidum | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Rickettsia prowazekii | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Borreliella burgdorferi | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Chlamydia sp. | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Campylobacter jejuni | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Helicobacter pylori | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | Pyrococcus abyssi | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Borreliella burgdorferi | - |
- |
- |
Campylobacter jejuni | - |
- |
- |
Chlamydia sp. | - |
enzyme found in three different species | - |
Dictyostelium discoideum | - |
- |
- |
Helicobacter pylori | Q9ZJ93 | - |
- |
Mycobacterium tuberculosis | - |
- |
- |
Pyrococcus abyssi | Q9UZ51 | - |
- |
Pyrococcus horikoshii | - |
- |
- |
Rickettsia prowazekii | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Treponema pallidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from transformed E. coli using His-tag | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Dictyostelium discoideum | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Saccharolobus solfataricus | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Pyrococcus horikoshii | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Treponema pallidum | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Rickettsia prowazekii | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Borreliella burgdorferi | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Chlamydia sp. | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Campylobacter jejuni | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Pyrococcus abyssi | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Dictyostelium discoideum | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Saccharolobus solfataricus | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Pyrococcus horikoshii | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Treponema pallidum | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Rickettsia prowazekii | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Borreliella burgdorferi | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Chlamydia sp. | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Campylobacter jejuni | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Pyrococcus abyssi | dTMP + tetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Dictyostelium discoideum | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Saccharolobus solfataricus | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Pyrococcus horikoshii | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Treponema pallidum | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Rickettsia prowazekii | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Borreliella burgdorferi | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Chlamydia sp. | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Campylobacter jejuni | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FMN | - |
? | |
5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Pyrococcus abyssi | dTMP + tetrahydrofolate + FMN | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 31000, gel filtration, SDS-PAGE | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Dictyostelium discoideum | |
FADH2 | - |
Mycobacterium tuberculosis | |
FADH2 | - |
Saccharolobus solfataricus | |
FADH2 | - |
Pyrococcus horikoshii | |
FADH2 | - |
Treponema pallidum | |
FADH2 | - |
Rickettsia prowazekii | |
FADH2 | - |
Borreliella burgdorferi | |
FADH2 | - |
Chlamydia sp. | |
FADH2 | - |
Campylobacter jejuni | |
FADH2 | - |
Pyrococcus abyssi | |
FADH2 | activity is dependent on reduced flavin nucleotides | Helicobacter pylori | |
FMNH2 | - |
Dictyostelium discoideum | |
FMNH2 | - |
Mycobacterium tuberculosis | |
FMNH2 | - |
Saccharolobus solfataricus | |
FMNH2 | - |
Pyrococcus horikoshii | |
FMNH2 | - |
Treponema pallidum | |
FMNH2 | - |
Rickettsia prowazekii | |
FMNH2 | - |
Borreliella burgdorferi | |
FMNH2 | - |
Chlamydia sp. | |
FMNH2 | - |
Campylobacter jejuni | |
FMNH2 | - |
Pyrococcus abyssi | |
FMNH2 | activity is dependent on reduced flavin nucleotides | Helicobacter pylori |