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Literature summary for 2.1.1.148 extracted from
- An alternative flavin-dependent mechanism for thymidylate synthesis (2002), Science, 297, 105-107.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | increases activity | Helicobacter pylori |  |
| FMN | increases activity | Helicobacter pylori | |
| UMP | - |
Helicobacter pylori | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Rickettsia prowazekii |
| medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Borreliella burgdorferi |
| medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Campylobacter jejuni |
| medicine | promising medical target, thyX is present in a number of pathogenic bacteria but absent in human | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| complemetation of Escherichia coli, transformants are able to grow in the absence of thymidine | Helicobacter pylori |
| complemetation of Escherichia coli, transformants are unable to grow in the absence of thymidine | Pyrococcus abyssi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S107A | no activity | Helicobacter pylori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Helicobacter pylori |
| 31500 | - |
calculated from DNA sequence | Helicobacter pylori |
| 111000 | - |
gel filtration | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Dictyostelium discoideum | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Mycobacterium tuberculosis | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Saccharolobus solfataricus | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Pyrococcus horikoshii | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Treponema pallidum | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Rickettsia prowazekii | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Borreliella burgdorferi | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Chlamydia sp. | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Campylobacter jejuni | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Helicobacter pylori | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | Pyrococcus abyssi | de novo synthesis of thymidylate | dTMP + tetrahydrofolate + FAD | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Borreliella burgdorferi | - |
- |
- |
| Campylobacter jejuni | - |
- |
- |
| Chlamydia sp. | - |
enzyme found in three different species | - |
| Dictyostelium discoideum | - |
- |
- |
| Helicobacter pylori | Q9ZJ93 | - |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Pyrococcus abyssi | Q9UZ51 | - |
- |
| Pyrococcus horikoshii | - |
- |
- |
| Rickettsia prowazekii | - |
- |
- |
| Saccharolobus solfataricus | - |
- |
- |
| Treponema pallidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from transformed E. coli using His-tag | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Dictyostelium discoideum | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Saccharolobus solfataricus | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Pyrococcus horikoshii | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Treponema pallidum | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Rickettsia prowazekii | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Borreliella burgdorferi | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Chlamydia sp. | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Campylobacter jejuni | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | - |
Pyrococcus abyssi | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Dictyostelium discoideum | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Saccharolobus solfataricus | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Pyrococcus horikoshii | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Treponema pallidum | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Rickettsia prowazekii | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Borreliella burgdorferi | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Chlamydia sp. | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Campylobacter jejuni | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Helicobacter pylori | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FADH2 | de novo synthesis of thymidylate | Pyrococcus abyssi | dTMP + tetrahydrofolate + FAD | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Dictyostelium discoideum | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Mycobacterium tuberculosis | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Saccharolobus solfataricus | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Pyrococcus horikoshii | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Treponema pallidum | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Rickettsia prowazekii | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Borreliella burgdorferi | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Chlamydia sp. | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Campylobacter jejuni | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Helicobacter pylori | dTMP + tetrahydrofolate + FMN | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP + FMNH2 | - |
Pyrococcus abyssi | dTMP + tetrahydrofolate + FMN | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 31000, gel filtration, SDS-PAGE | Helicobacter pylori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FADH2 | - |
Dictyostelium discoideum | |
| FADH2 | - |
Mycobacterium tuberculosis | |
| FADH2 | - |
Saccharolobus solfataricus | |
| FADH2 | - |
Pyrococcus horikoshii | |
| FADH2 | - |
Treponema pallidum | |
| FADH2 | - |
Rickettsia prowazekii | |
| FADH2 | - |
Borreliella burgdorferi | |
| FADH2 | - |
Chlamydia sp. | |
| FADH2 | - |
Campylobacter jejuni | |
| FADH2 | - |
Pyrococcus abyssi | |
| FADH2 | activity is dependent on reduced flavin nucleotides | Helicobacter pylori | |
| FMNH2 | - |
Dictyostelium discoideum | |
| FMNH2 | - |
Mycobacterium tuberculosis | |
| FMNH2 | - |
Saccharolobus solfataricus | |
| FMNH2 | - |
Pyrococcus horikoshii | |
| FMNH2 | - |
Treponema pallidum | |
| FMNH2 | - |
Rickettsia prowazekii | |
| FMNH2 | - |
Borreliella burgdorferi | |
| FMNH2 | - |
Chlamydia sp. | |
| FMNH2 | - |
Campylobacter jejuni | |
| FMNH2 | - |
Pyrococcus abyssi | |
| FMNH2 | activity is dependent on reduced flavin nucleotides | Helicobacter pylori | |
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