BRENDA - Enzyme Database
show all sequences of 2.1.1.147

The formation of corydaline and related alkaloids in Corydalis cava in vivo and in vitro

Rueffer, M.; Bauer, W.; Zenk, M.H.; Can. J. Chem. 72, 170-175 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Corydalis cava
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
S-adenosyl-L-methionine
-
Corydalis cava
0.005
-
Berberine
-
Corydalis cava
0.016
-
NADPH
-
Corydalis cava
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Corydalis cava
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
gel filtration
Corydalis cava
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corydalis cava
-
-
-
Purification (Commentary)
Commentary
Organism
-
Corydalis cava
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bulb
-
Corydalis cava
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Corydalis cava
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + berberine + NADPH + H+
-
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
S-adenosyl-L-methionine + columbamine + NADPH + H+
27% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
-
-
-
?
S-adenosyl-L-methionine + coptisine + NADPH + H+
21% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
-
-
-
?
S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + thalictricavine + NADP+
-
485156
Corydalis cava
?
S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
67% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
-
-
-
?
S-adenosyl-L-methionine + palmatine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Corydalis cava
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
Corydalis cava
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Corydalis cava
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
Corydalis cava
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0026
-
S-adenosyl-L-homocysteine
-
Corydalis cava
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Corydalis cava
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0026
-
S-adenosyl-L-homocysteine
-
Corydalis cava
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
S-adenosyl-L-methionine
-
Corydalis cava
0.005
-
Berberine
-
Corydalis cava
0.016
-
NADPH
-
Corydalis cava
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Corydalis cava
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
gel filtration
Corydalis cava
Purification (Commentary) (protein specific)
Commentary
Organism
-
Corydalis cava
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bulb
-
Corydalis cava
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Corydalis cava
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + berberine + NADPH + H+
-
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
S-adenosyl-L-methionine + columbamine + NADPH + H+
27% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
-
-
-
?
S-adenosyl-L-methionine + coptisine + NADPH + H+
21% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
-
-
-
?
S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + thalictricavine + NADP+
-
485156
Corydalis cava
?
S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
67% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
-
-
-
?
S-adenosyl-L-methionine + palmatine + NADPH + H+
93% of the activity with berberine
485156
Corydalis cava
S-adenosyl-L-homocysteine + corydaline + NADP+
-
485156
Corydalis cava
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Corydalis cava
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
Corydalis cava
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Corydalis cava
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
Corydalis cava
Other publictions for EC 2.1.1.147
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485156
Rueffer
-
The formation of corydaline an ...
Corydalis cava
Can. J. Chem.
72
170-175
1994
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1
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6
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1
1
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1
1
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1
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1
1
3
1
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1
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1
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1
1
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6
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1
1
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1
1
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