Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pimpinella anisum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2.5 mM, 97% inhibition | Pimpinella anisum | |
Fe2+ | 2.5 mM, 47% inhibition | Pimpinella anisum | |
Mn2+ | 2.5 mM, 35% inhibition | Pimpinella anisum | |
additional information | not inhibitory: 2.5 mM K+, Na+, Ca2+, and Mg2+ | Pimpinella anisum | |
Zn2+ | 2.5 mM, 97% inhibition | Pimpinella anisum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0193 | - |
isoeugenol | pH 7.5, 25°C | Pimpinella anisum | |
0.0545 | - |
S-adenosyl-L-methionine | pH 7.5, 25°C | Pimpinella anisum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pimpinella anisum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | expression throughout the plant with highest level in developing fruit | Pimpinella anisum | - |
additional information | expression throughout the plant with highest level in developing fruit | Pimpinella anisum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: chavicol, eugenol, guaiacol, caffeic acid, 2-propylphenol | Pimpinella anisum | ? | - |
? | |
S-adenosyl-L-methionine + 5-methoxyeugenol | about 18% of the activity with isoeugenol | Pimpinella anisum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + dihydrochavicol | about 23% of the activity with isoeugenol | Pimpinella anisum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + dihydroeugenol | about 50% of the activity with isoeugenol | Pimpinella anisum | S-adenosyl-L-homocysteine + methyldihydroeugenol | - |
? | |
S-adenosyl-L-methionine + isoeugenol | methylation of the p-OH group | Pimpinella anisum | S-adenosyl-L-homocysteine + methylisoeugenol | - |
? | |
S-adenosyl-L-methionine + t-anol | methylation of the p-OH group. 45% of the rate with isoeugenol | Pimpinella anisum | S-adenosyl-L-homocysteine + t-anethole | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AIMT1 | - |
Pimpinella anisum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for at least 30 min | Pimpinella anisum |
37 | - |
stable for at least 30 min | Pimpinella anisum |
50 | - |
30 min, 88% residual activity | Pimpinella anisum |
65 | - |
30 min, less than 5% residual activity | Pimpinella anisum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
isoeugenol | pH 7.5, 25°C | Pimpinella anisum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Pimpinella anisum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Pimpinella anisum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
isoeugenol | pH 7.5, 25°C | Pimpinella anisum |