Literature summary for 2.1.1.146 extracted from

  • Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
    Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain (2009), Plant Physiol., 149, 384-394.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pimpinella anisum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2.5 mM, 97% inhibition Pimpinella anisum
Fe2+ 2.5 mM, 47% inhibition Pimpinella anisum
Mn2+ 2.5 mM, 35% inhibition Pimpinella anisum
additional information not inhibitory: 2.5 mM K+, Na+, Ca2+, and Mg2+ Pimpinella anisum
Zn2+ 2.5 mM, 97% inhibition Pimpinella anisum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0193
-
isoeugenol pH 7.5, 25°C Pimpinella anisum
0.0545
-
S-adenosyl-L-methionine pH 7.5, 25°C Pimpinella anisum

Organism

Organism UniProt Comment Textmining
Pimpinella anisum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression throughout the plant with highest level in developing fruit Pimpinella anisum
-
fruit expression throughout the plant with highest level in developing fruit Pimpinella anisum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: chavicol, eugenol, guaiacol, caffeic acid, 2-propylphenol Pimpinella anisum ?
-
?
S-adenosyl-L-methionine + isoeugenol methylation of the p-OH group Pimpinella anisum S-adenosyl-L-homocysteine + methylisoeugenol
-
?
S-adenosyl-L-methionine + 5-methoxyeugenol about 18% of the activity with isoeugenol Pimpinella anisum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + dihydrochavicol about 23% of the activity with isoeugenol Pimpinella anisum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + dihydroeugenol about 50% of the activity with isoeugenol Pimpinella anisum S-adenosyl-L-homocysteine + methyldihydroeugenol
-
?
S-adenosyl-L-methionine + t-anol methylation of the p-OH group. 45% of the rate with isoeugenol Pimpinella anisum S-adenosyl-L-homocysteine + t-anethole
-
?

Synonyms

Synonyms Comment Organism
AIMT1
-
Pimpinella anisum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable for at least 30 min Pimpinella anisum
50
-
30 min, 88% residual activity Pimpinella anisum
65
-
30 min, less than 5% residual activity Pimpinella anisum
20
-
stable for at least 30 min Pimpinella anisum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
isoeugenol pH 7.5, 25°C Pimpinella anisum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Pimpinella anisum

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Pimpinella anisum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.78
-
isoeugenol pH 7.5, 25°C Pimpinella anisum