BRENDA - Enzyme Database
show all sequences of 2.1.1.144

A novel methyltransferase catalyzes the methyl esterification of trans-aconitate in Escherichia coli

Cai, H.; Clarke, S.; J. Biol. Chem. 274, 13470-13479 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in a Escherichia coli BL21
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
trans-aconitate
-
Escherichia coli
5
-
DL-isocitrate
-
Escherichia coli
7.1
-
citrate
-
Escherichia coli
9.1
-
(2R,3S)-isocitrate
-
Escherichia coli
33
-
cis-aconitate
-
Escherichia coli
45
-
citrate
-
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Escherichia coli
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + cis-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
S-adenosyl-L-methionine + citrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + DL-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + trans-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in a Escherichia coli BL21
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
trans-aconitate
-
Escherichia coli
5
-
DL-isocitrate
-
Escherichia coli
7.1
-
citrate
-
Escherichia coli
9.1
-
(2R,3S)-isocitrate
-
Escherichia coli
33
-
cis-aconitate
-
Escherichia coli
45
-
citrate
-
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Escherichia coli
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + cis-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
S-adenosyl-L-methionine + citrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + DL-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + trans-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
Other publictions for EC 2.1.1.144
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288663
Cai
Identification of the gene and ...
Escherichia coli
Biochemistry
40
13699-13709
2001
-
-
-
-
-
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4
1
-
-
1
-
2
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-
1
-
-
-
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-
5
-
-
-
-
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-
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-
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-
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4
1
-
-
1
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-
1
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-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288664
Cai
Distinct reactions catalyzed b ...
Escherichia coli
Biochemistry
40
2210-2219
2001
-
-
-
-
-
-
1
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288662
Cai
A novel methyltransferase cata ...
Escherichia coli
J. Biol. Chem.
274
13470-13479
1999
-
-
1
-
-
-
-
6
1
-
-
1
-
5
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
1
-
-
1
-
-
-
1
-
-
-
-
5
-
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-
-
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-
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