Application | Comment | Organism |
---|---|---|
degradation | the amino acids of Region 1 provide a tight substrate orientation imposed by hydrophobic interactions between the sterol side chain and the SMT active site contacts and control the production and processing of the transmethylation pathways governed by the first and second C1-transfer activities | Glycine max |
Cloned (Comment) | Organism |
---|---|
overexpression of the pET vector in Escherichia coli BL21(DE3) | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
F82I | continues to catalyze strongly both the first and second C1-transfer activities and generates product sets similar to the control | Glycine max |
F82L | first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity | Glycine max |
F85L | first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity | Glycine max |
F91L | first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity | Glycine max |
F93L | first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity | Glycine max |
W87L | first and second C1-transfer activities are greatly reduced from the control activities | Glycine max |
Y83F | first C1-transfer activity is relatively unaffected whereas the second C1-transfer activity generates different amounts of product compared to the control | Glycine max |
Y83F | Km and kcat, of wild-type enzyme and mutant enzyme are similar for cycloartenol as substrate | Glycine max |
Y83L | first C1-transfer activity greatly reduced from the control activity, loss of the second C1-transfer activity | Glycine max |
Y83L | Km and kcat, of wild-type enzyme and mutant enzyme are similar for cycloartenol as substrate | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
Cycloartenol | - |
Glycine max | |
0.045 | - |
Cycloartenol | wild-type and mutants Y83F and Y83L | Glycine max | |
0.05 | - |
24(28)-methylene lophenol | wild-type | Glycine max | |
0.05 | - |
24(28)-methylene lophenol | mutant Y83F | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
wild-type and mutants Y83F and Y83L, gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24(28)-methylene lophenol + S-adenosyl-L-methionine | - |
Glycine max | ? | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Glycine max | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + cycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + (24R)-24-methylcycloart-25-en-3beta-ol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMT | - |
Glycine max |
sterol methyltransferase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
24(28)-methylene lophenol | wild-type | Glycine max | |
0.005 | - |
24(28)-methylene lophenol | mutant Y83F | Glycine max | |
0.01 | - |
Cycloartenol | - |
Glycine max | |
0.01 | - |
Cycloartenol | wild-type and mutants Y83F and Y83L | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.9 | - |
Glycine max |